Midori Green Nucleic Acid Stain
Ethidium bromide (EB) is most commonly used nucleic acid stain in molecular biology laboratories. It has been proved to be strong carcinogen and therefore considered hazardous for laboratory personnel and environment.
Midori Green DNA stain is a nucleic acid stain which can be used as a safer alternative to the traditional Ethidium bromide stain for detecting nucleic acid in agarose gels. It is as sensitive as Ethidium bromide and can be used exactly the same way in agarose gel electrophoresis.
Figure 1: Aarose gels,stained by Midori Green DNA Stain (A) and Ethidium bromide (B).
Midori Green DNA Stain was tested in the mouse bone marrow micronucleus test to evaluate the genotoxic potential of this new dye. The result was negative (in contrast to Ethidiumbromide and other dyes ) Furthermore the dye was applied to a chromosome aberration test. We can state that Midori Green is non-carcinogenic and according to the Ames test it causes significantly fewer mutations than Ethidium bromide (Figure 2). If you like to get more information, please download the safety report.
Figure 2: Summary of Ames test results. Mutagenicity ratio of Midori Green DNA Stain and Ethidium bromide with metabolic activation (S9) in TA98 mutagenicity assay: a dose-response. According to the results of the Ames test, Midori Green DNA Stain is significantly less mutagenic compared to Ethidium bromide.
Notes: The thickness of gel should be <0.5cm. Repeated melting of gels containing Midori Green DNA Stain may result in low sensitivity. Midori Green DNA Stain is non-carcinogenic but may irritate skin and eyes. Please wear gloves while handling.
Midori Green is not classified as hazardous waste, thus can be safely disposed of down the drain or as regular trash. Waste must be disposed of in accordance with federal, state and local environmental control regulations.
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