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Midori Green Nucleic Acid Stain

Midori Green Nucleic Acid Stain

Product Description

Ethidium bromide (EB) is most commonly used nucleic acid stain in molecular biology laboratories. It has been proved to be strong carcinogen and therefore considered hazardous for laboratory personnel and environment.

Midori Green DNA stain is a nucleic acid stain which can be used as a safer alternative to the traditional Ethidium bromide stain for detecting nucleic acid in agarose gels. It is as sensitive as Ethidium bromide and can be used exactly the same way in agarose gel electrophoresis.

(A)                                                           (B)

mg-ebr

Figure 1: Aarose gels,stained by Midori Green DNA Stain (A) and Ethidium bromide (B).

 

Safety

Midori Green DNA Stain was tested in the mouse bone marrow micronucleus test to evaluate the genotoxic potential of this new dye. The result was negative (in contrast to Ethidiumbromide and other dyes ) Furthermore the dye was applied to a chromosome aberration test. We can state that Midori Green is non-carcinogenic and according to the Ames test it causes significantly fewer mutations than Ethidium bromide (Figure 2). If you like to get more information, please download the safety report.

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Figure 2: Summary of Ames test results. Mutagenicity ratio of Midori Green DNA Stain and Ethidium bromide with metabolic activation (S9) in TA98 mutagenicity assay: a dose-response. According to the results of the Ames test, Midori Green DNA Stain is significantly less mutagenic compared to Ethidium bromide.



Protocol


  • Prepare 100ml of agarose gel solution (concentration from 0.8-3.0%) and heat until the solution is completely clear and no small floating particles are visible
  • Add 2-5µl of Midori Green DNA Stain to the gel solution and mix it gently
  • Optional: cool the gel to 50-60ºC and cast the gel into the gel tray
  • When the gel is solid, load the samples and perform electrophoresis
  • Detect the bands under UV illuminator or blue light

Notes: The thickness of gel should be <0.5cm. Repeated melting of gels containing Midori Green DNA Stain may result in low sensitivity. Midori Green DNA Stain is non-carcinogenic but may irritate skin and eyes. Please wear gloves while handling.


Disposal

Midori Green is not classified as hazardous waste, thus can be safely disposed of down the drain or as regular trash. Waste must be disposed of in accordance with federal, state and local environmental control regulations.


FAQ

What is the shelf life of Midori Green ?
2 years
What is the shipment/storage temperature ?
Room temperature
Can Midori Green be diluted ?
Midori Green solution can be diluted in the ultrapure water
Can we use Midori Green for RNA as well as DNA ?
Midori Green can be used for RNA as well as dsDNA and ssDNA
Is Midori Green suitable for UV illumination ?
Yes, Midori Green is suitable for UV illumination
Is it possible to use Midori Green on acrylamide gels ?
No, Midori Green is recommended to use with agarose gels
Can Midori Green be used with TAE and TBE buffers ?
Yes
Is it possible to stain DNA with Midori Green in agarose gels as with EtBr (ethidium bromide) ?
Yes, it can be used with the DNA in the gel like EtBr. However, post-staining should give a better sensitivity than precast gels, eliminating any possibility for the dye to interfere with the migration and thus the separation of the nucleic acid bands
Can Midori Green be used for post-staining ?
Yes
How should Midori Green be diluted when used for post-staining ?
Add 5-15µl of Midori Green in 100ml of buffer solution. Adjust optimal concentration and staining duration (from 5 to 60 minutes usually) according to your experiment
Does Midori Green migrate in the gel ?
Yes, therefore it is recommended to use optimal electrophoresis time and % of agarose running gels. For longer products/running time we recommend to use post-staining
What are the excitation and emmision lengths of Midori Green ?
Midori Green has two secondary fluorescence excitation peaks (~300nm; ~400nm) and one strong excitation peak centered around 500nm. The fluorescence emission is centered around 540nm
Which filters (wavelength) can be used for Midori Green?
The filters designed for green dyes (like GFP/SYBR green filter) are the most sensitive. However, EtBr filter and simply UV-light without any filter can be used as well.
Is it possible to add Midori Green when agarose is dissolved in TAE buffer with on the hot plate and magnetic stirrer during the heating process ?
It is not recommended to add Midori Green in hot agarose solution. We recommend after making agarose solution, to cool it down to approximately 60°C, then add Midori Green, shake carefully and mix thoroughly
Does Midori Green perform with low concentration of DNA ?
Yes, Midori Green can perform with low concentration of DNA. However, smaller fragments of less than 300 bp may not be as bright as the larger ones (post-staining could improve the results)
Does Midori Green interfere with downstream applications ?
Avoid leaving Midori Green in downstream solution. You may purify the DNA solution with a purification kit
Can Midori Green be used for pulse field gel electrophoresis ?
It is not recommended to use Midori Green for pulse field gel electrophoresis
Is it possible to use Midori Green in Southern blot experiments ?
Yes, Midori Green is perfectly compatible with Southern blotting experiments
Does the stain interfere with recovery of DNA from the stained gel once it is run ?
No, the stain should not cause any problems for further experiments with that DNA
Where should Midori Green be stored ?
For the maximum performance and stability Midori Green should be stored in the dark (Midori Green is provided in brown vials)
Does Midori Green penetrate the latex gloves ?
Midori Green should be impenetrable to latex gloves. However, as in common laboratory practice, it is recommended to wear gloves while working with Midori Green
How can Midori Green be disposed after use ?
Midori Green does not create any toxic waste. Therefore, it can be disposed according to laboratory regulations


Packaging Description


  • 1ml Midori Green 50'000X
Ref#: MG02

Availability

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