Midori Green Nucleic Acid Stain

Product Description

Ethidium bromide (EB) is most commonly used nucleic acid stain in molecular biology laboratories. It has been proved to be strong carcinogen and therefore considered hazardous for laboratory personnel and environment.

Midori Green DNA stain is a nucleic acid stain which can be used as a safer alternative to the traditional Ethidium bromide stain for detecting nucleic acid in agarose gels. It is as sensitive as Ethidium bromide and can be used exactly the same way in agarose gel electrophoresis.

Safety

Midori Green DNA Stain was tested in the mouse bone marrow micronucleus test to evaluate the genotoxic potential of this new dye. The result was negative (in contrast to Ethidiumbromide and other dyes ) Furthermore the dye was applied to a chromosome aberration test. We can state that Midori Green is non-carcinogenic and according to the Ames test it causes significantly fewer mutations than Ethidium bromide (Figure 2). If you like to get more information, please download the safety report.

Protocol

• Prepare 100ml of agarose gel solution (concentration from 0.8-3.0%) and heat until the solution is completely clear and no small floating particles are visible
• Add 2-5µl of Midori Green DNA Stain to the gel solution and mix it gently
• Optional: cool the gel to 50-60ºC and cast the gel into the gel tray
• When the gel is solid, load the samples and perform electrophoresis
• Detect the bands under UV illuminator or blue light
  

Notes: The thickness of gel should be <0.5cm. Repeated melting of gels containing Midori Green DNA Stain may result in low sensitivity. Midori Green DNA Stain is non-carcinogenic but may irritate skin and eyes. Please wear gloves while handling.

Disposal

Midori Green is not classified as hazardous waste, thus can be safely disposed of down the drain or as regular trash. Waste must be disposed of in accordance with federal, state and local environmental control regulations.

FAQ

Packaging Description