KAPA Library Preparation Kit (Illumina series) 10rxns

Product Description

The KAPA Library Preparation Kit provides all of the enzymes and reaction buffers required for constructing libraries from fragmented dsDNA via the following steps:

• End repair: produce blunt-ended, 5’-phosphorylated fragments
• A-tailing: add dAMP to the 3’-ends of the dsDNA library fragments
• Adaptor ligation: ligate dsDNA adaptors with 3’-dTMP overhangs to library fragments
• Library amplification: PCR amplification of library fragments carrying appropriate adaptor sequences on both ends.

Reaction buffers are supplied in convenient, concentrated “master mix” formats comprising all of the required reaction components except oligonucleotide adaptors or PCR primers.

High fidelity PCR is used to selectively enrich library fragments carrying appropriate adaptor sequences and to amplify the amount of DNA prior to sequencing. KAPA HiFi DNA Polymerase is designed for low bias, high fidelity PCR representing the method of choice for the amplification of nextgeneration sequencing libraries. The intrinsic high processivity of the enzyme results in significant improvements in yield, sensitivity, speed, target length and the ability to amplify difficult templates.

This kit is primarily intended for the construction of Illumina libraries, but may be used for other applications requiring efficient end-repair, A-tailing, ligation, and/or library amplification steps like:

• Genomic DNA libraries
• Paired-end DNA libraries
• Paired-end multiplexed (indexed/barcoded) DNA libraries

Packaging Description