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KAPA2G™ Robust HotStart ReadyMix with dye 500rxns

KAPA2G™ Robust HotStart ReadyMix with dye 500rxns

Product Description

The KAPA2G Robust HotStart is recommended for all standard end-point PCR assays, particularly those in which wild-type Taq DNA polymerase does not perform satisfactorily. The kit is based on KAPA2G Robust HotStart DNA Polymerase, a highly robust and versatile engineered enzyme that was derived from Taq DNA polymerase using the KAPA Biosystems molecular evolution platform. The novel amino acid mutations in KAPA2G Robust HotStart DNA Polymerase offer higher processivity and specific activity, which translates to robust performance across a wide range of GC- and AT-rich templates and amplicons, as well as improved tolerance to common PCR inhibitors.

KAPA2G Robust HotStart is designed for the amplification of DNA fragments up to 6 kb in standard end-point PCR assays from a wide variety of templates. It is particularly suited for:

  • amplification from templates with a high GC- or AT- content
  • templates containing common PCR inhibitors (e.g. salts, urea, SDS, ethanol...)
  • colony PCR
  • optimization of low yield or low specificity assays
  • direct PCR on cells without purification
  • genome closure (sequencing)
  • routine PCR

Packaging Description


  • 6.25ml KAPA2G™ Robust HotStart ReadyMix 2X with green dye
  • 500rxns (25μl)

Ref#: KK5705


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