KAPA2G™ Fast HotStart 500U (without dNTPs, MgCl2 free buffer)

Evolved specifically for speed and high performance, KAPA2G Fast HotStart offers Fast PCR based on the intrinsic ability of the KAPA2G Fast HotStart DNA polymerase to synthesize DNA at a much faster rate than wild-type polymerases. As little as 1 second per kilobase extension rate allows for increased productivity and faster time to results without sacrificing performance.

Any existing PCR assay performed efficiently with wild-type Taq polymerase may be converted to a Fast PCR assay with KAPA2G Fast HotStart DNA Polymerase. Typically, very little re-optimization of reaction parameters is required. Fast PCR assays with KAPA2G Fast HotStart may be performed with any conventional Peltier-based thermocycler and thin-walled PCR tubes or plates.

KAPA2G Fast HotStart PCR Kits are designed for the fast amplification of DNA fragments up to 5kb in standard end-point PCR assays, using reaction volumes up to 25μl. Amplicons generated with these kits are suitable for routine downstream applications, such as:

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