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KAPA2G™ Robust PCR Kit 100U (without dNTPs)

KAPA2G™ Robust PCR Kit 100U (without dNTPs)

Product Description

The KAPA2G Robust PCR Kit is recommended for all standard end-point PCR assays, particularly those in which wild-type Taq DNA polymerase does not perform satisfactorily. The kit is based on KAPA2G Robust DNA Polymerase, a highly robust and versatile engineered enzyme that was derived from Taq DNA polymerase using the KAPA Biosystems molecular evolution platform. The novel amino acid mutations in KAPA2G Robust DNA Polymerase offer higher processivity and specific activity, which translates to robust performance across a wide range of GC- and AT-rich templates and amplicons, as well as improved tolerance to common PCR inhibitors.

KAPA2G Robust PCR Kits are designed for the amplification of DNA fragments up to 6 kb in standard end-point PCR assays from a wide variety of templates. It is particularly suited for:

  • amplification from templates with a high GC- or AT- content
  • templates containing common PCR inhibitors (e.g. salts, urea, SDS, ethanol...)
  • colony PCR
  • optimization of low yield or low specificity assays
  • direct PCR on cells without purification
  • genome closure (sequencing)

Packaging Description


  • KAPA2G™ Robust DNA Polymerase (5U/μl)
  • 5X KAPA2G Buffer A (containing MgCl2)
  • 5X KAPA2G Buffer B (containing MgCl2)
  • 5X KAPA2G GC Buffer (containing MgCl2)
  • 5X KAPA Enhancer 1
  • 25mM MgCl2
  • 100 units / 200rxns (25μl)

Ref#: KK5023


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Instrumentation

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