KAPATaq HotStart

KAPATaq HotStart improves the specificity and sensitivity in PCR

KAPATaq HotStart DNA Polymerase is based on the single- subunit, wild-type Taq DNA polymerase of the thermophilic bacterium Thermus aquaticus. In the HotStart formulation, the enzyme (which is purified from recombinant E. coli) is combined with a proprietary antibody that inactivates the enzyme until the first denaturation step. This eliminates spurious amplification products resulting from non-specific priming events during reaction setup and initiation, and increases overall reaction efficiency and sensitivity. Antibody-based hotstart PCR enzymes offer superior performance compared to hotstart formulations based on chemical modification of the enzyme, as enzyme re-activation is faster, more complete and is optimal at pH values which are also optimal for polymerase activity.

KAPATaq HotStart DNA Polymerase has 5’→3’ polymerase and 5’→3’ exonuclease activities, but no 3’→5’ exonuclease (proofreading) activity. The enzyme has an error rate of approximately 1 error per 2.2 x 10⁵ nucleotides incorporated. PCR products generated with KAPATaq HotStart are A-tailed and may be cloned into TA cloning vectors.

KAPATaq HotStart DNA Polymerase offers:

• Improved PCR specificity
• Improved PCR sensitivity
• Higher yields
  
• Convenience - assembly of PCR reactions at room temperature
  
• Short activation time