Roche 454 FLX/Lib-A

Accurate quantification of the number of amplifiable molecules in a fragmented library is critical to the outcome of sequencing results on next-generation sequencing platforms. Over and underestimation of library concentration result in suboptimal sequencing capacity. qPCR is widely regarded as the gold standard for accurate quantification of DNA libraries as it is the only technique capable of measuring the number of amplifiable molecules.

Accurate qPCR-based library quantification ultimately depends on three factors:

the accuracy and reproducibility of the standards used
the ability of the DNA polymerase used in the qPCR to amplify all adaptor-flanked molecules with equal efficiency
accurate and reproducible liquid handling

KAPA Library Quantification Kits are rigorously tested to ensure minimal lot-to-lot variation. In addition, KAPA SYBR^® FAST qPCR Kits are designed for high performance, high-throughput, real-time PCR.

KAPA Library Quantification Kits Brochure

Broad Institute NGS Case Study

454 FLX/Lib-A Library Quantification Kit(Universal qPCR) 500rxns

500 reactions (20μl) - including Primer Premix and DNA Standards for Roche 454 FLX/Lib-A libraries and Fast Real-Time PCR Mix (2X) intended for use on all real-time instruments

Ref#: KK4820

454 FLX Library Quantification Kit Technical Sheet.pdf (216,67 KB)

454 FLX/Lib-A Library Quantification Kit(ABI Prism™qPCR) 500rxns

500 reactions (20μl) - including Primer Premix and DNA Standards for Roche 454 FLX/Lib-A libraries and Fast Real-Time PCR Mix (2X) for ABI Prism™ Real-Time PCR instruments (except 7500)

Ref#: KK4830

454 FLX Library Quantification Kit Technical Sheet.pdf (216,67 KB)

454 FLX/Lib-A Library Quantification Kit (Bio-Rad™ qPCR) 500rxns

500 reactions (20μl) - including Primer Premix and DNA Standards for Roche 454 FLX/Lib-A libraries and Fast Real-Time PCR Mix (2X) for Bio-Rad iCycler™ serie Real-Time PCR instruments

Ref#: KK4840

454 FLX Library Quantification Kit Technical Sheet.pdf (216,67 KB)

454 FLX/Lib-A Library Quantification Kit (LC®480 qPCR) 500rxns

500 reactions (20μl) - including Primer Premix and DNA Standards for Roche 454 FLX/Lib-A libraries and Fast Real-Time PCR Mix (2X) optimized for Roche LightCycler® 480

Ref#: KK4850

454 FLX Library Quantification Kit Technical Sheet.pdf (216,67 KB)