Library preparation
KAPA Library Preparation Kits provide all of the enzymes and reaction buffers required for constructing libraries from fragmented dsDNA via the following steps:
1. End repair: produce blunt-ended, 5’-phosphorylated fragments
2. A-tailing: add dAMP to the 3’-ends of the dsDNA library fragments
3. Adaptor ligation: ligate dsDNA adaptors with 3’-dTMP overhangs to library fragments
4. Amplification: PCR amplification of library fragments carrying appropriate adaptor sequences on both ends
This kit is primarily intended for the construction of Illumina libraries, but may be used for other applications requiring efficient end-repair, A-tailing, ligation, and/or library amplification steps:
- Genomic DNA libraries
- Paired-end DNA libraries
- Paired-end multiplexed (indexed/barcoded) DNA libraries
KAPA Library Preparation Kit (Illumina series) 10rxns
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10 reactions
The KAPA Library Preparation Kit provides all of the enzymes and reaction buffers required for constructing libraries from fragmented dsDNA Ref#: KK8200
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KAPA Library Preparation Kit (Illumina series) 50rxns
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50 reactions
The KAPA Library Preparation Kit provides all of the enzymes and reaction buffers required for constructing libraries from fragmented dsDNA Ref#: KK8201
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KAPA HTP Library Preparation Kit (Illumina series) 96rxns
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• Automation friendly format - 96 libraries per kit/run
• Improved DNA recovery • Reduced cost of SPRI beads by 60% • Higher yields of adaptor-ligated library molecules • Reduced PCR bias resulting in improved sequencing coverage Ref#: KK8234
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KAPA Real-Time PCR Library Preparation Kit (ILMN series) 10rxns
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10 reactions
The KAPA Real-Time PCR Library Preparation Kit provides all of the enzymes and reaction buffers required for constructing libraries from fragmented dsDNA Ref#: KK8220
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KAPA Real-Time PCR Library Preparation Kit (ILMN series) 50rxns
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50 reactions
The KAPA Real-Time PCR Library Preparation Kit provides all of the enzymes and reaction buffers required for constructing libraries from fragmented dsDNA Ref#: KK8221
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