Library amplification

High fidelity PCR is used to selectively enrich library fragments carrying appropriate adaptor sequences and to amplify the amount of DNA prior to sequencing. During PCR enrichment, the polymerase does not synthesize all library fragments with equal efficiency. This amplification bias exacerbates uneven sequence coverage.

KAPAHiFi™ Library Amplification Kits have been designed to address PCR-induced bias. Kits contain the novel KAPAHiFi™ DNA Polymerase, engineered for high fidelity and processivity and capable of balanced amplification of complex library DNA.

KAPAHiFi™ Library Amplification Kits are ideally suited to the amplification of complex genomic libraries and enrichment of targeted capture sequences where: Low amplification bias is required in order to maintain genome representation High fidelity is required to identify single nucleotide polymorphisms (SNPs)		Benefits of performing high fidelity real-time PCR for next-generation sequencing library amplification include: Real-time monitoring of amplification allows precise control over the optimal number of PCR cycles Real-time amplification workflows are amenable to automation Real-time amplification plots provide quality metrics for individual enriched libraries, eliminating expensive and time-consuming post-enrichment gel electrophoresis and identifying inconsistencies in library preparation Seamless integration with KAPA Library Quantification Kits