Accurate quantification of the number of amplifiable molecules in a fragmented library is critical to the outcome of sequencing results on next-generation sequencing platforms. Over and underestimation of library concentration result in suboptimal sequencing capacity. qPCR is widely regarded as the gold standard for accurate quantification of DNA libraries as it is the only technique capable of measuring the number of amplifiable molecules.
Accurate qPCR-based library quantification ultimately depends on three factors:
KAPA Library Quantification Kits are rigorously tested to ensure minimal lot-to-lot variation. In addition, KAPA SYBR® FAST qPCR Kits are designed for high performance, high-throughput, real-time PCR.
KAPA Library Quantification Kit for Roche 454 FLX/Lib-A information>>
KAPA Library Quantification Kit for Roche 454 Titanium/Lib-L information>>
KAPA Library Quantification Kit for ABI SOLiD™ series information>>
KAPA Library Quantification Kit for Illumina information>>
KAPA Library Quantification Kit for Ion Torrent information>>
Delivering unsurpassed data quality for 40-cycle runs in as little as 40 minutes, the Eco™ system revolutionizes qPCR accessibility for both new and experienced Real-Time PCR users :