PCR enzymes

Expand your PCR possibilities with KAPA Biosystems Next-Generation DNA polymerases

The majority of today’s research and biomedical applications were designed around a small subset of native enzymes isolated from nature over 20 years ago, and many of today’s reagents still contain these same enzymes, virtually unmodified. Kapa Biosystems is not just another life science reagents supplier. Their high-throughput molecular evolution platform allows this company to engineer and tailor enzymes for specific applications.

Next-generation PCR is defined as the shift from generalist wild-type enzymes toward designer specialist enzymes. This transition is enabled by Kapa Biosystems’ powerful protein engineering platform. This platform makes now possible transformation of wild-type enzymes into engineered enzymes through a process of high-throughput molecular evolution.

KAPA Enzyme Selection Guide

Next-Generation PCR enzymes:

KAPAHiFi™ DNA Polymerase exhibits over 30x increased fidelity as compared to wild-type Taq polymerase:
	KK2101 KAPAHiFi™ DNA Polymerase 100U KK2102 KAPAHiFi™ DNA Polymerase 250U KK2601 KAPAHiFi™ HotStart ReadyMix 100rxns KK2602 KAPAHiFi™ HotStart ReadyMix 500rxns KK2501 KAPAHiFi™ HotStart DNA Polymerase 100U KK2502 KAPAHiFi™ HotStart DNA Polymerase 250U
KAPA2G™ Fast and Fast HotStart are the ultimate hot start enzymes for extreme speed and performance:
	KK5102 KAPA2G™ Fast ReadyMix with loading dye 500rxns KK5009 KAPA2G™ Fast PCR Kit 250U KK5021 KAPA2G™ Fast PCR Kit 250U (without dNTPs) KK5801 KAPA2G™ Fast Multiplex PCR Mix 100rxns KK5802 KAPA2G™ Fast Multiplex PCR Mix 500rxns KK5603 KAPA2G™ Fast HotStart ReadyMix 100rxns KK5601 KAPA2G™ Fast HotStart ReadyMix 500rxns KK5502 KAPA2G™ Fast HotStart 250U KK5500 KAPA2G™ Fast HotStart 500U KK5503 KAPA2G™ Fast HotStart 250U (without dNTPs) KK5501 KAPA2G™ Fast HotStart 500U (without dNTPs) KK5512 KAPA2G™ Fast HotStart 250U (MgCl2 free buffer) KK5510 KAPA2G™ Fast HotStart 500U (MgCl2 free buffer) KK5513 KAPA2G™ Fast HotStart 250U (without dNTPs, MgCl2 free buffer) KK5511 KAPA2G™ Fast HotStart 500U (without dNTPs, MgCl2 free buffer)
KAPA Mouse Genotyping Kits allows for the reliable extraction and amplification of DNA fragments from mouse tissue in 1 hour:
	KK7302 KAPA Mouse Genotyping Kits 500rxns KK7352 KAPA Mouse Genotyping Kits HotStart 500rxns
KAPA2G™ Robust and Robust HotStart significantly improve difficult PCR success rates:
	KK5004 KAPA2G™ Robust PCR Kit 100U KK5005 KAPA2G™ Robust PCR Kit 250U KK5023 KAPA2G™ Robust PCR Kit 100U (without dNTPs) KK5024 KAPA2G™ Robust PCR Kit 250U (without dNTPs) KK5701 KAPA2G™ Robust HotStart ReadyMix 100rxns KK5702 KAPA2G™ Robust HotStart ReadyMix 500rxns KK5516 KAPA2G™ Robust HotStart 250U KK5518 KAPA2G™ Robust HotStart 500U KK5515 KAPA2G™ Robust HotStart 250U (without dNTPs) KK5517 KAPA2G™ Robust HotStart 500U (without dNTPs)
KAPA Plant PCR Kit for direct PCR on crude samples from a variety of plant species and tissue types:
	KK7251 KAPA Plant PCR Kits 250U KK7252 KAPA Plant PCR Kits 500U
KAPALongRange™ DNA Polymerase has been engineered for long templates and extreme sensitivity:
	KK3006 KAPALongRange™ DNA Polymerase 100U KK3005 KAPALongRange™ DNA Polymerase 250U KK3501 KAPALongRange™ HotStart DNA Polymerase 100U KK3502 KAPALongRange™ HotStart DNA Polymerase 250U KK3503 KAPALongRange™ HotStart DNA Polymerase 500U
KAPA Blood PCR Mix eliminates the need for purification with the first polymerase engineered for PCR direct from blood:
	KK7002 KAPA Blood PCR Mix for fluorescent assays 500rxns KK7004 KAPA Blood PCR Mix for fluorescent assays 1000rxns KK7003 KAPA Blood PCR Mix for agarose gel analysis 500rxns KK7005 KAPA Blood PCR Mix for agarose gel analysis 1000rxns