Blood PCR

KAPA Blood PCR Kits contain a second-generation DNA polymerase evolved specifically for DNA amplification directly from blood, in a choice of two optimized, easy-to-use PCR mixes

KAPA Blood PCR kits eliminate the need for purification before PCR.

KAPA Blood PCR Kits contain KAPA Blood DNA Polymerase, a second-generation enzyme derived through a process of molecular evolution and the first DNA polymerase engineered specifically for the amplification of DNA directly from whole blood. The enzyme is available in two optimized, easy-to-use 2x PCR mixes, which contain all components required for Whole Blood PCR, except primers and template (blood). Using KAPA Blood PCR Kits, DNA fragments may be amplified directly from reactions containing 1 - 20% (v/v) whole human blood without pretreatment of blood samples or DNA isolation, significantly reducing contamination risk, turnaround time and cost of genetic testing.

KAPA Blood PCR Mix A is preferred for assays employing highly sensitive fluorescent detection systems. KAPA Blood Mix B is formulated for higher yields and GC-rich amplicons and is recommended when analysis is by agarose gel electrophoresis and ethidium bromide staining. Products generated with KAPA Blood PCR Mixes A or B may be analyzed by restriction endonuclease digestion, DNA sequencing or dHPLC analysis.