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KAPA2G Fast HotStart

KAPA2G™ Fast HotStart PCR Kits contain a second generation DNA polymerase engineered for extreme speed


The superior speed and performance of the KAPA2G™ Fast HotStart PCR Kit is attributable to KAPA2G™ Fast HotStart DNA Polymerase, a novel, engineered enzyme derived from Taq DNA polymerase using the KAPA Biosystem's molecular evolution platform. Designed specifically for this purpose, the KAPA2G™ Fast Hotstart PCR Kit offers Fast PCR that is not merely based on the reduction of cycling times, but on the intrinsicability of the KAPA2G™ Fast HotStart DNA Polymerase to synthesize DNA at a much faster rate.

As second-generation polymerase for the ultimate in speed and performance, KAPA2G™ Fast HotStart DNA Polymerase and optimized buffer system offers :

  • Reductions in total PCR reaction times from 20% to more than 70%
  • High speed without compromising performance
  • Extension rate as low as 1 second per kilobase
  • No specific consumables or instrumentation required

In this formulation, the enzyme is combined with a proprietary antibody that inactivates the enzyme until the first denaturation step. This eliminates spurious amplification products resulting from non-specific priming events during reaction setup and initiation, and increases overall reaction efficiency and sensitivity.

KAPA Mouse Genotyping Kits HotStart Brochure

KAPA2G™ Fast Multiplex PCR Mix 100rxns

KAPA2G™ Fast Multiplex PCR Mix 100rxns
100 reactions (25μl)
Ready to use PCR Master Mix (containing MgCl2)
Ideal for multiplexing
Ref#: KK5801

KAPA2G™ Fast Multiplex PCR Mix 500rxns

KAPA2G™ Fast Multiplex PCR Mix 500rxns
500 reactions (25μl)
Ready to use PCR Master Mix (containing MgCl2)
Ideal for multiplexing
Ref#: KK5802

KAPA2G™ Fast HotStart ReadyMix 100rxns

KAPA2G™ Fast HotStart ReadyMix 100rxns
100 reactions (25μl)
Ready to use PCR Master Mix (containing MgCl2)
Ref#: KK5603

KAPA2G™ Fast HotStart ReadyMix 500rxns

KAPA2G™ Fast HotStart ReadyMix 500rxns
500 reactions (25μl)
Ready to use PCR Master Mix (containing MgCl2)
Ref#: KK5601

KAPA2G™ Fast HotStart 250U

KAPA2G™ Fast HotStart 250U
500 reactions (25μl) including dNTPs
Core kit
Buffer containing MgCl2
Ref#: KK5502

KAPA2G™ Fast HotStart 500U

KAPA2G™ Fast HotStart 500U
1000 reactions (25μl) including dNTPs
Core kit
Buffer containing MgCl2
Ref#: KK5500

KAPA2G™ Fast HotStart 250U (without dNTPs)

KAPA2G™ Fast HotStart 250U (without dNTPs)
500 reactions (25μl)
Buffer containing MgCl2
Core kit, dNTPs not supplied
Ref#: KK5503

KAPA2G™ Fast HotStart 500U (without dNTPs)

KAPA2G™ Fast HotStart 500U (without dNTPs)
1000 reactions (25μl)
Buffer containing MgCl2
Core kit, dNTPs not supplied
Ref#: KK5501

KAPA2G™ Fast HotStart 250U (MgCl2 free buffer)

KAPA2G™ Fast HotStart 250U (MgCl2 free buffer)
500 reactions (25μl) including dNTPs
Core kit
MgCl2 free buffer
Ref#: KK5512

KAPA2G™ Fast HotStart 500U (MgCl2 free buffer)

KAPA2G™ Fast HotStart 500U (MgCl2 free buffer)
1000 reactions (25μl) including dNTPs
Core kit
MgCl2 free buffer
Ref#: KK5510

KAPA2G™ Fast HotStart 500U (without dNTPs, MgCl2 free buffer)

KAPA2G™ Fast HotStart 500U (without dNTPs, MgCl2 free buffer)
1000 reactions (25μl)
MgCl2 free buffer
Core kit, dNTPs not supplied
Ref#: KK5511

KAPA Mouse Genotyping Kit HotStart 500rxns

KAPA Mouse Genotyping Kit HotStart 500rxns
500 reactions (25μl)
Starting from ear or tail tissue
15' incubation
45' PCR
Gel electrophoresis
Ref#: KK7352

KAPA2G Fast HotStart Genotyping Mix 500rxns

KAPA2G Fast HotStart Genotyping Mix 500rxns
500 reactions (25μl)
45' HotStart PCR for quick mouse genotyping
Ref#: KK5621