KAPALongRange HotStart

KAPALongRange™ HotStart DNA Polymerase has been engineered for long templates and extreme sensitivity

The KAPALongRange HotStart system is engineered for the amplification of long and complex targets up to 20kb. The system is optimized specifically for high yields from limiting starting DNA and exhibits extreme sensitivity. KAPALongRange HotStart also exhibits a 4x improvement in fidelity as compared to standard Taq DNA polymerase.

The majority of PCR products from a KAPALongRange™ HotStart DNA Polymerase reaction have a single deoxyadenosine added to the 3' ends of the amplicon and can be used for ligation into single 3'-T-overhang cloning sites.

• Amplification of PCR products as large as 30kb (plasmid)
• Higher fidelity than Taq polymerase
• Ability to amplify long templates from limiting complex genomic DNA

In the HotStart formulation, the enzyme is combined with a proprietary antibody that inactivates the enzyme until the first denaturation step. This eliminates spurious amplification products resulting from non-specific priming events during reaction setup and initiation, and increases overall reaction efficiency and sensitivity.

KapaLongRange Hotstart Brochure