KAPA SYBR® FAST LightCycler® 480

The reaction efficiency of qPCR is critical for accurate and reliable gene quantification. Wild type Taq DNA polymerase is often inhibited by real-time PCR reaction conditions, leading to significant loss in specificity and sensitivity of the assay. The KAPA SYBR^® FAST DNA Polymerase has been engineered to perform optimally in stringent qPCR reaction conditions, exhibiting dramatic improvements in signal-to-noise ratio (fluorescence), quantification cycle (Cq), linearity, and sensitivity. The novel DNA polymerase and proprietary buffer system enhances the amplification efficiency of difficult templates, including both GC and AT-rich templates.
High speed is achieved through the increased processivity of the engineered KAPA SYBR^® FAST DNA Polymerase and the short initial denaturation time required for the activation of the proprietary antibody-mediated hot start.