Winner of a 2016 R&D 100 Award, the FEMTO Pulse Automated Pulsed-Field CE Instrument is shattering analytic barriers for nucleic acid separation and detection. Using a pulsed-field power supply, the FEMTO Pulse is the first ever parallel capillary electrophoresis instrument able to resolve DNA smears and DNA fragments through 200,000 bp.
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The FEMTO Pulse is a versatile instrument with various applications across many research disciplines. Capable of separating large DNA fragments and smears, BAC clones, total RNA, and small RNA with unmatched speed, the list of potential applications of the FEMTO Pulse goes on and on. The researchers’ imagination is the only limiting factor for the FEMTO Pulse.
|Maximum sample throughput||12 samples per run|
|Maximum unattended sample capacity||288 samples|
|Minimum sample volume||20 µl of liquid for injection; as little as 1-2 µl of actual sample required|
|Quantitative kits||NGS Library Preparations for Illumina and PacBio, Genomic DNA, cfDNA, Total RNA, mRNA, ribodepleted RNA, microRNA, and DNA fragments|
|Sizing accuracy||Typically 5% or better|
|Detection sensitivity||As low as 5 fg/µl for fragments and 200 fg/µl for a smear|
|Light source||2 x 700 mA, 10W LED, 460 nm excitation wavelength|
|Detector||High sensitivity CCD; 500-600 nm emission wavelength|
|Software||FEMTO Pulse Operational Software, PROSize® 3.0 Data Analysis Software|
|Data export format||CSV, PDF, flexible numerical or binary output options|
|Environmental conditions||Indoor use, normal laboratory environment 20-23°C|
|Relative humidity range||<80% (non-condensing)|
|Electrical||100-200 VAC; 50-60 Hz; 15A (alternate configurations available)|
|Dimensions (W x D x H)||38.0 x 51.0 x 61.0 cm|
The FEMTO Pulse Automated Pulsed-Field CE Instrument has innumerable applications and supports researcher innovation. With sensitivity in the femtogram range and sizing through 200,000 bp, new measurements can be made and novel applications developed. The FEMTO Pulse is poised to have a significant impact on various applications including :
The FEMTO Pulse does not require amplification with fluorescently-labeled probes to achieve a remarkable level of detection sensitivity, instead a sensitive intercalating dye is used to detect DNA and RNA. This strategy allows the FEMTO Pulse to analyze nucleic acids without prior knowledge of the samples; in turn broadening the analytic scope of the instrument. What could you do with an instrument as sensitive and powerful as the FEMTO Pulse ?