Library Quantification Kits for Next-Generation Sequencing
Next-Generation Sequencing Pipeline
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Accurate quantification of the number of amplifiable molecules in a fragmented library is critical to the outcome of sequencing results on next-generation sequencing platforms. Over and underestimation of library concentration result in suboptimal sequencing capacity. qPCR is widely regarded as the gold standard for accurate quantification of DNA libraries as it is the only technique capable of measuring the number of amplifiable molecules. The broad dynamic range of qPCR also enables accurate quantification of dilute libraries where genetic material is limiting (e.g. unamplified array eluates from a sequence capture experiment). Accurate qPCR-based library quantification ultimately depends on three factors:
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