Fast PCR of multiple DNA fragments

KAPA2G™ Fast Multiplex PCR Kit

Multiplex PCR is a challenging application that typically requires more optimization than standard, single amplicon PCR assays. The key to successful Multiplex PCR is the ability to define a single set of reaction parameters (reagent concentrations and cycling parameters) that allows for all primers to anneal with high specificity to their target sequences and be extended with the same efficiency. Primer design, as well as the enzyme and buffer system, are critical factors in this challenge.

The KAPA2G Fast Multiplex PCR Kit is ideally suited for:

• typing of transgenic organisms
• amplification of microsatellites
• typing and detection of pathogens
• amplification of multiple DNA fragments for SNP genoptyping