KAPA HiFi Library Amplification Kits for NGS

KAPAHiFi™ Library Amplification Kits

High fidelity PCR is used to selectively enrich library fragments carrying appropriate adaptor sequences and to amplify the amount of DNA prior to sequencing. During PCR enrichment of libraries, it is critical that amplification bias is kept to a minimum to ensure uniform sequence coverage. This enables efficient sequencing where the highest overall coverage can be achieved from the least amount of total sequence. KAPA HiFi™ Library Amplification Kits are designed for low bias, high fidelity PCR representing the method of choice for the amplification of next-generation sequencing libraries.

KAPAHiFi™ HotStart ReadyMix (2X) is ideally suited to the amplification of complex genomic libraries and enrichment of targeted capture sequences where:

• Low amplification bias is required in order to maintain genome representation
• High fidelity is required to identify single nucleotide polymorphisms (SNPs)

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