KAPA Library Preparation Kits contain an engineered polymerase for reduced bias and improved coverage
|
|
KAPA Library Preparation Kits provide all of the enzymes and reaction buffers required for constructing libraries from fragmented dsDNA via the following steps: 1. End repair: produce blunt-ended, 5’-phosphorylated fragments 2. A-tailing: add dAMP to the 3’-ends of the dsDNA library fragments 3. Adaptor ligation: ligate dsDNA adaptors with 3’-dTMP overhangs to library fragments 4. Amplification: PCR amplification of library fragments carrying appropriate adaptor sequences on both ends. |
