Accel-AmpliconView larger

Accel-Amplicon™ Custom NGS Panels

AACP

Let’s Design Your Targeted Sequencing Assays

Accel-Amplicon Custom NGS Panels offer a completely curated, targeted NGS workflow to rapidly and precisely interrogate genomic targets relevant to your research. This approach is ideal as a follow-up to whole genome or exome sequencing studies, as well as diving deep into specific biological pathways.

Features :

  • Flexible, intelligent assay design
  • Simple and scalable
  • Reliable
  • Informative

More details

Description
Related products
Resources

Description

Accelerating variant discovery in challenging biological samples

Accel-Amplicon Custom NGS Panels generate highly multiplexed, targeted PCR libraries that are compatible with both Illumina® and Ion Torrent™ sequencing platforms. The unique single-tube design is also compatible with most sample types, including limited or degraded samples such as FFPE and cfDNA samples.

Tailored to meet your specific needs, Accel-Amplicon Custom NGS Panels can be customized to include coverage of both clinically-relevant hotspot loci and regions of contiguous coverage, as well as Sample_ID targets spiked-in at a low percentage (2-4% of reads). This allows for somatic mutation detection using high depth of coverage and sample identification using low coverage depth of germline targets.

Benefits

  • Enables discovery, characterization, and screening of SNPs and small indels (< 15 bp).
  • Provides comprehensive coverage of critical hotspot SNVs, indels, and contiguous tiled regions in coding regions and intron-exon boundaries.
  • Tailors your assay at the size that works best for you — from a small panel of just 15 amplicons up to 1,500 — all in a single-tube multiplexed reaction.
  • Generates complete libraries in under 2 hours with a fast, easy workflow.
  • Delivers highly reproducible performance from only 10-25 ng input DNA, including fragmented or degraded samples.
  • Produces highly specific, exceptional target coverage uniformity to make your sequencing reads work for you.

Workflow

The Accel-Amplicon workflow provides a fast, single-tube approach resulting in a < 2-hour start-to-finish procedure, including hands-on time.

The single-tube workflow includes two brief incubations to generate the multiplex amplicon targets and add a unique combination of Illumina-compatible indexed adapters, creating up to 96 uniquely-indexed libraries for multiplexing on a single sequencing run.

Simplified Data Analysis

Eliminate the variant valling bottleneck
In partnership with Genialis, Swift provides basic and clinical researchers’ with a streamlined data analysis solution that integrates alignment, primer trimming, QC and variant calling into one, simple pipeline.

Please contact us for more details.

Resources