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SensiFAST SYBR Lo-ROX Kit (QuantStudio)

BIO94005

SensiFAST SYBR® Lo-ROX Kit has been developed for fast, highly reproducible real-time PCR and has been validated on commonly used real-time instruments.  This version is specifically designed for QuantStudio instruments.

The latest advances in buffer chemistry and enhancers, together with an antibody-mediated hot-start DNA polymerase system, ensure that the SensiFAST SYBR® Lo-ROX Kit produces fast, highly-specific and ultra-sensitive real-time PCR.

Features :

  • Rapid - hot-start capability saves time and reduces primer-dimer formation
  • Unique buffer chemistry - for highest specificity
  • Broad dynamic range - for high sensitivity
  • Simple and reproducible - just add primers and template

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More details

Description
Applications
Specifications
Related products
Resources

Description

SensiFAST SYBR® Lo-ROX Kit is designed for fast, highly sensitive and reproducible real-time PCR and has been validated on commonly used real-time instruments. The antibody-mediated hot-start DNA polymerase system reduces the chances of primer/dimers formation.

This allows a much greater dynamic range by removing competition for reaction components during amplification, in turn leading to greater sensitivity. The addition of the latest advances in buffer chemistry and enhancers also ensures that the SensiFAST SYBR® Lo-ROX Kit produces highly-specific and reproducible real-time PCR results in under 30 minutes. For expression analysis, SensiFAST cDNA Synthesis Kit is the perfect partner for SensiFAST SYBR® Lo-ROX Kit for very fast cDNA synthesis and real-time results, without compromising sensitivity or quality.

For ease-of-use, SensiFAST SYBR® Lo-ROX is provided as a 2X mastermix containing all the components necessary for real-time PCR, including the SYBR® Green I dye, dNTPs, stabilizers and enhancers. SensiFAST SYBR® Lo-ROX contains premixed ROX for optional use as a passive reference.

Instrument Compatibility

ABI/Thermo Fisher Scientific 7500, 7500 FAST, ViiA7™, Quantstudio™ 3,5,6,7,12k Flex, Stratagene (Agilent) Mx4000™, Mx3000P™, Mx3005P™ (see Specifications tab for product selection table), as well as other instruments that do not require the use of a passive reference.

Specifications

Applications

  • Biomarker discovery and validation
  • ChIP
  • Drug therapy efficacy
  • Gene knockdown validation
  • Microarray validation
  • Pathogen detection
  • Cancer risk assessment
  • Detection of extremely low copy targets
  • Gene dosage determination
  • Genotyping, allelic discrimination, SNP, haplotyping
  • Microbial quantification
  • DNA damage measurement
  • Gene expression analysis
  • High‑throughput qPCR
  • Mitochondrial DNA studies

Resources

Product Guide

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