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SensiFAST Probe Lo-ROX Kit (QuantStudio)

BIO84005

SensiFAST™ Probe Lo-ROX Kit has been developed for fast, highly reproducible real-time PCR and has been validated on commonly used real-time PCR instruments. It is specifically designed for QuantStudio instruments. A combination of the latest advances in buffer chemistry and PCR enhancers, together with a hot-start DNA polymerase, ensures that SensiFAST Probe Kit delivers fast, highly-specific and ultra-sensitive real-time PCR.

Features :

  • Highly specific - minimal non-specific activity leading to better efficiency and greater accuracy
  • Ultra-sensitive - perfect for difficult and low copy number samples
  • Efficient multiplexing - increasing throughput and conserving precious sample material
  • Fast - optimized for fast cycling conditions, ideal for high-throughput

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More details

Description
Applications
Specifications
Related products
Resources

Description

SensiFAST™ Probe Lo-ROX Kit has been developed for fast, highly reproducible real-time PCR and has been validated on commonly used real-time instruments. The kit has been formulated for use with probe-detection technology, including TaqMan®, Scorpions® and molecular beacon probes.

The kit is optimized for fast mode on fast qPCR instruments and fast cycling conditions on standard qPCR instruments. A combination of the latest advances in buffer chemistry and PCR enhancers, together with a hot-start DNA polymerase, ensures that the SensiFAST Probe Kit delivers shorter run times, is highly reproducible, highly-specific and ultra-sensitive, allowing more samples to be run with the highest confidence, ideal for high-throughput assays.

The advanced buffer chemistry and enhancers also make SensiFAST Probe perfect for multiplexing. SensiFAST Probe Lo-ROX is provided as a 2X mastermix containing all the components necessary for real-time PCR, including dNTPs, stabilizers and enhancers.

For expression analysis, SensiFAST cDNA Synthesis Kit is the perfect partner for SensiFAST Probe Lo-ROX Kit for very fast cDNA synthesis and real-time results, without compromising sensitivity or quality. SensiFAST Probe Lo-ROX Kit contains premixed ROX for optional use.

Instrument Compatibility

ABI/Thermo Fisher Scientific 7500, 7500 FAST, ViiA7™, Quantstudio™ 3,5,6,7,12k Flex, Stratagene (Agilent) Mx4000™, Mx3000P™, Mx3005P™ (see Specifications tab for product selection table), as well as other instruments that do not require the use of a passive reference.

Specifications

Applications

  • Biomarker discovery and validation
  • ChIP
  • Drug therapy efficacy
  • Gene knockdown validation
  • Microarray validation
  • Pathogen detection
  • Cancer risk assessment
  • Detection of extremely low copy targets
  • Gene dosage determination
  • Genotyping, allelic discrimination, SNP, haplotyping
  • Microbial quantification
  • DNA damage measurement
  • Gene expression analysis
  • High‑throughput qPCR
  • Mitochondrial DNA studies

Resources

Product Guide

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