RainDrop Digital PCR System

2004400

The RainDrop® Digital PCR System is the most sensitive and precise platform for nucleic acids detection and monitoring of cell-free and cell-based biomarkers for cancer, viruses, pathogens or infectious diseases.

Features :

  • Detects 1 mutant amongst 250,000 wild-type molecules with a lower limit of detection of 1 in 1,000,000
  • Multiplexing : conducts several tests together on the same sample
  • Real-time closed-loop control of droplet creation ensures uniformity both within and across runs
  • Closed-tube design : ensures highest quality by eliminating contamination or carryover

More details

Description
Applications
Specifications
Resources

Description

What is Digital PCR ?

Divide and count : single volume divided into countable-size partitions.

Digital counting of each molecule with an endpoint PCR assay provides sensitive determination of absolute concentration.

Millions of droplets matter

A Billion reactions. Digital answers. Experience the final frontier of PCR.

Capable of generating more than a billion reactions in a single day, the RainDrop® Digital PCR System surpasses all existing technologies and establishes a new performance standard in sensitivity, quantitation and multiplexing. Based on RainDance’s proven digital droplet platform, the RainDrop System fundamentally changes the performance of molecular assays by enabling digital answers across a number of important applications including low-frequency tumor allele detection, gene expression, copy number variation, and SNP measurement.

Innovative design

A new gold standard in sensitivity is only possible through the careful consideration of every aspect of the system. The RainDrop System offers a number of innovative digital droplet-based design advantages :

  • Automated delivery of filtered oil from sealed on-board reservoirs eliminates any contamination risks from the environment or pipetting
  • Real-time closed-loop control of droplet creation ensures uniformity both within and across runs
  • Automated filling of PCR tubes with emulsion avoids possible losses with manual pipetting techniques
  • All wetted surfaces are single use, eliminating the potential for any carryover of droplets from one sample to the next that can occur with reusable fluid path approaches
  • Sealed amplicon containment completely eliminates catastrophic contamination issues from spills and other problems with pierced foil designs
  • Logical channel-to-channel workflow, integrated bar-coding and sample tracking through the RainDrop software eliminate the risk of experiment disasters caused by simple reformatting mistakes in sample handling

Robust and reliable platform

Don’t risk your research experiments to first-generation designs. The RainDrop System is the third generation of RainDance’s digital droplet-based PCR instrumentation and uses the fourth generation of our microfluidic chips. These innovations have a long track record of success in the lab and have been detailed by leading research institutions in publications including Analytical Chemistry, Lab on a Chip, and PNAS.

Streamlined workflow

The RainDrop System shifts the PCR paradigm from a single color per marker approach to a more scalable and precise multi-color and intensity-per-marker method. This novel approach increases sensitivity by generating between 1 million and 10 million pico-liter sized droplets per lane, which is a 500 – 10,000X improvement over existing PCR methods. Since each droplet encapsulates a single molecule, researchers can quickly determine the absolute number of droplets containing specific target DNA and compare that to the amount of droplets with normal, background wild-type DNA.

Sensitivity

To assess the lower limit of detection (LLOD) of an EGFR mutation, varying concentrations of mutant were diluted into genomic wild-type DNA. The assay delivered a linear response (R2 = 0.998) down to 1 mutant amongst 250'000 wild-type molecules, with a LLOD of 1 in more than 1,000,000 as defined by the average of the wild-type only controls plus 3X the standard deviation.

Multiplexing

By adjusting concentrations of the probes for each individual assay, 4 mutations plus wild-type were measured simultaneously with just VIC and FAM fluorophores. Multiplexing can be expanded to higher plex levels, and it enables detection, identification, and measurement of multiple possible mutations from a single DNA sample.

Droplet creation on RainDrop Source

-> 80,000 PCR reactions per second – droplets are created in all 8 channels simultaneously at rates approaching 5 million per minute

-> CCD vision system provides real-time closed-loop control to ensure droplet size uniformity

-> Droplet data retained in Source log files for traceability

Specifications

Detection channelsFAM & VIC
Sensitivity1:250,000
LLODMore than 1:1,000,000
Capacity8 lanes/chip
Maximum sample throughput24 samples/day
SoftwareGraphical user interface provides intuitive operation
Operating temperature range15-30° C
Operating temperature fluctuation<0.3°C/minute
Humidity5-85% RH, non-condensing
Required compressed air input pressure range80-100 psi (0.55 MPa – 0.69 MPa)
Dimensions (W x D x H)26.0 x 37.0 x 43.0 cm, each instrument
Voltage/frequency/power120-230 VAC / 50-60 Hz / 100W

Applications

  • Rare event detection, including somatic mutations in cancer samples, in tumour and circulating tumour DNA and circulating faetal DNA in maternal blood samples
  • Pathogen detection including RNA and DNA viruses, bacteria and fungi in both mammalian blood and tissue samples as well as plant and seed materials
  • Copy Number Variation in both mammalian blood samples as well as plant and seed materials. The RainDrop Digital PCR System has the ability to differentiate between 8 and 9 copies of a single gene
  • Analysis of DNA samples, including FFPE quality/integrity and quantity
  • Gene expression measurement by one-step or two-step PCR, including microRNA
  • Methylated DNA biomarkers quantification
  • NGS library quantification
  • NGS target enrichment
  • Sequencing result validation (MAF)
  • ThunderBolts™ Cancer and Myeloid NGS Panels targeting many known cancer genes

Resources