The RainDrop® Digital PCR System is the most sensitive and precise platform for nucleic acids detection and monitoring of cell-free and cell-based biomarkers for cancer, viruses, pathogens or infectious diseases.
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Divide and count : single volume divided into countable-size partitions.
Digital counting of each molecule with an endpoint PCR assay provides sensitive determination of absolute concentration.
A Billion reactions. Digital answers. Experience the final frontier of PCR.
Capable of generating more than a billion reactions in a single day, the RainDrop® Digital PCR System surpasses all existing technologies and establishes a new performance standard in sensitivity, quantitation and multiplexing. Based on RainDance’s proven digital droplet platform, the RainDrop System fundamentally changes the performance of molecular assays by enabling digital answers across a number of important applications including low-frequency tumor allele detection, gene expression, copy number variation, and SNP measurement.
A new gold standard in sensitivity is only possible through the careful consideration of every aspect of the system. The RainDrop System offers a number of innovative digital droplet-based design advantages :
Don’t risk your research experiments to first-generation designs. The RainDrop System is the third generation of RainDance’s digital droplet-based PCR instrumentation and uses the fourth generation of our microfluidic chips. These innovations have a long track record of success in the lab and have been detailed by leading research institutions in publications including Analytical Chemistry, Lab on a Chip, and PNAS.
The RainDrop System shifts the PCR paradigm from a single color per marker approach to a more scalable and precise multi-color and intensity-per-marker method. This novel approach increases sensitivity by generating between 1 million and 10 million pico-liter sized droplets per lane, which is a 500 – 10,000X improvement over existing PCR methods. Since each droplet encapsulates a single molecule, researchers can quickly determine the absolute number of droplets containing specific target DNA and compare that to the amount of droplets with normal, background wild-type DNA.
To assess the lower limit of detection (LLOD) of an EGFR mutation, varying concentrations of mutant were diluted into genomic wild-type DNA. The assay delivered a linear response (R2 = 0.998) down to 1 mutant amongst 250'000 wild-type molecules, with a LLOD of 1 in more than 1,000,000 as defined by the average of the wild-type only controls plus 3X the standard deviation.
By adjusting concentrations of the probes for each individual assay, 4 mutations plus wild-type were measured simultaneously with just VIC and FAM fluorophores. Multiplexing can be expanded to higher plex levels, and it enables detection, identification, and measurement of multiple possible mutations from a single DNA sample.
-> 80,000 PCR reactions per second – droplets are created in all 8 channels simultaneously at rates approaching 5 million per minute
-> CCD vision system provides real-time closed-loop control to ensure droplet size uniformity
-> Droplet data retained in Source log files for traceability
|Detection channels||FAM & VIC|
|LLOD||More than 1:1,000,000|
|Maximum sample throughput||24 samples/day|
|Software||Graphical user interface provides intuitive operation|
|Operating temperature range||15-30° C|
|Operating temperature fluctuation||<0.3°C/minute|
|Humidity||5-85% RH, non-condensing|
|Required compressed air input pressure range||80-100 psi (0.55 MPa – 0.69 MPa)|
|Dimensions (W x D x H)||26.0 x 37.0 x 43.0 cm, each instrument|
|Voltage/frequency/power||120-230 VAC / 50-60 Hz / 100W|