Electrophoresis 101 MiniLab

Ideal for students as a hands-on introductory lab to electrophoresis. With this MiniLab, students cast their own gels and run real DNA. Results can be visualized within a 45 minute class period, eliminating need for multiple sessions. Students also learn how to do a semi-log plot of known vs unknown samples.

• Give your students the complete hands-on experience of casting, loading, running, documenting, and analyzing electrophoresis gels
• Students will develop an understanding of gel electrophoresis principles by observing separation of dye and DNA samples
• Students use their data to create a standard curve for DNA fragments of known molecular weight the calculate the weight of unknown DNA fragments
• An essential introduction to electrophoresis and one of our most popular labs
• Store in the refrigerator; GelGreen™ Cups should be left in the original package and protected from light; guaranteed stable for six months with proper storage

Materials included in each MiniLab

Each MiniLab contains enough materials for 10 workstations, 2 – 3 students per workstation.

• 10 1% agarose GelGreen™ Cups
• 3 color dye samples and 3 DNA samples
• 1 bottle of 100 ml Tris-Borate-EDTA (TBE) buffer concentrate, enough to make 2L of 1X running buffer
• 1 bag of 0.65 ml microcentrifuge tubes
• 1 bag of 2-200 µl micropipette tips
• 1 Teacher's guide

Teaching resources

Teacher's guide, student guide and classroom handouts to get the most out of your Electrophoresis 101 Kit.