Qsep400 Multi-Channel Capillary Electrophoresis System

C400100

The Qsep400™ has the highest throughput in the Qsep family. It is designed as a 4-capillaries gel electrophoresis (CGE) system, integrated with a high sensitivity LED-induced fluorescence (LEDIF) detector with an easily-replaceable 4-channel gel-cartridge and 96-well auto-sampler, which can analyze/detect four samples at the same time.

Features :

  • Run 4 - 96 samples in a row
  • Resolves fragments from 15 bp to 15,000 bp
  • Get resolution down to 2-3 bp for fragments
  • Detection starts at 5 pg/μl

More details

Description
Applications
Specifications
Related products
Resources

Description

The Qsep400 has been designed for the larger research and clinical laboratories, who have large sample volumes. The high-throughput system provides high-speed analysis, superior results for all kinds of molecular genetic analysis applications.

The Qsep400, with variety of replaceable gel-cartridge kits, is specifically designed for DNA, RNA and Protein analysis providing reproducible and accurate test results in minutes.

The Qsep400 comes with Q-Analyzer software which has a simple-to-use User Interface (UI) that delivers consistent and high-precision data analysis and data export functions helping users in their daily molecular biology experiments.

Technology

Capillary electrophoresis (CE) is an instrumental approach to gel-electrophoresis, whose greatest advantage is its diverse range of applications. CE technology is commonly used by the biotech labs as a reliable, high resolution, and a highly sensitive tool. It is applied for protein, carbohydrate & DNA-related analysis such as oligonucleotides analysis, DNA sequencing, and dsDNA fragments analysis.

The instrumentation needed to perform capillary electrophoresis is relatively simple. A basic schematic of a capillary electrophoresis system is shown in figure. The system's main components are a sample vial, source and destination vials, a capillary, electrodes, a high-voltage power supply, a detector, and a data output and handling device.

The source vial, destination vial and capillary are filled with an electrolyte such as an aqueous buffer solution. To introduce the sample, the capillary inlet is placed into a vial containing the sample and then returned to the source vial (sample is introduced into the capillary via capillary action, pressure, or siphoning). The migration of the analytes is then initiated by an electric field that is applied between the source and destination vials and is supplied to the electrodes by the high-voltage power supply.

It is important to note that all ions, positive or negative, are pulled through the capillary in the same direction by electroosmotic flow. The analytes separate as they migrate due to their electrophoretic mobility, and are detected near the outlet end of the capillary. The output of the detector is sent to a data output and handling device such as an integrator or computer. The data is then displayed as an electropherogram, which reports detector response as a function of time. Separated chemical compounds appear as peaks with different migration times in an electropherogram.

CGE uses separation based on the difference in solute size as the particles migrate through the gel. Gels are useful because they minimize solute diffusion that causes zone broadening, prevent the capillary walls from absorbing the solute, and limit the heat transfer by slowing down the molecules.  A commonly used gel apparatus for the separation of proteins is capillary SDS-PAGE.  It is a highly sensitive system and only requires a small amount of sample.

BiOptic's CGE technology is a vertical electrophoresis system with a novel fluorescence detection :

Qsep cartridge schematic.jpg

Specifications

Automated sampling
4~96 samples
Capillary type
Disposable 4-channel gel cartridges
Analysis time
2~7 min / 4 samples
Resolution1~4 bp (<500 bp)
Sensitivity                                   
5 pg/μl
Minimum sample volume
1 μl
DetectionFluorescence
Light source
LED
Separation voltage
1~15 kV
SoftwareQ-Analyzer
ConnectionUSB
Power source
AC 100 - 240V
Weight26 kg
Dimensions (W x D x H)54.0 x 40.0 x 36.0 cm

Applications

  • Next-Generation Sequencing (NGS) Library Prep QC
  • RNA sample QC
  • CRISPR (Clusters of Regularly Interspaced Short Palindromic Repeats)
  • cfDNA (Circulating Cell Free DNA ) analysis
  • Chromatin immunoprecipitation (ChIP)
  • Genomic DNA (gDNA) analysis
  • SSR/microsatellites analysis
  • Oligonucleotide analysis
  • Plasmid analysis
  • RFLP (Restriction Fragment Length Polymorphism)
  • SNP (Single-nucleotide polymorphism) Genotyping 
  • Fluorescence detection of protein
  • Glycan
  • Immuno assay

Resources

Application Note - NGS Library QC

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Application Note - High Reslution

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