Pippin Prep

Targeted size selection for Next-Gen Sequencing (100 bp – 1.5 kb)

The Pippin Prep facilitates library construction for the most popular NGS platforms, and is recommended by Illumina and Ion Torrent for certain workflows. The platform features the ability to collect narrow and even fragment distributions, as well as the flexibility to collect wide ranges of fragments with minimal effort. Target sizes or ranges of sizes are entered in software, and fractions are collected in buffer. Up to 5 samples per gel cassette may be run, with no possibility of cross contamination.

Pre-cast agarose gel cassettes for DNA size selection

Gel cassettes for DNA selections are available in four agarose concentrations to provide a full range of size selection options between 100 bp and 1.5 kb, with limited capability up to 8 kb. For smaller fragment sizes, internal standards may be used for higher accuracy and increased sample capacity (from 4 to 5 samples per cassette). Run times are as little as 30 minutes. View the Pippin Prep cassette reference chart to explore the DNA size selection options and run times.

• 3% agarose, 100 - 250 bp
• 2% agarose, 100 - 600 bp
• 1.5% agarose, 250 bp – 1.5 kb

Workflow summary

• Paired-end sequencing
• miRNA isolation
• DNA sequencing
• RNA-seq
• Genotyping
• ChIP-seq
• Mate-pair sequencing
• emPCR