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PippinHT

HTP0001

The PippinHT increases throughput to 24 samples per run, compared to a maximum of five with Pippin Prep or Blue Pippin.

Features :

  • Sample-to-sample reproducibility streamlines research
  • Higher sample quality improves downstream analysis
  • 24 sample capacity meets high throughput sequencing demands

More details

Description
Applications
Specifications
Related products
Resources

Description

High Throughput Sizing for Next-Gen Sequencing

The PippinHT incorporates Sage Science’s highly regarded sizing technology and increases throughput to 24 samples per run, compared to a maximum of five with Pippin Prep or Blue Pippin. The instrument offers an efficient, automated solution for scientists running busy sequencing labs, core facilities, and other laboratories producing large-scale sequencing data.

Pre-cast agarose gel cassettes for DNA size selection

Gel cassettes kit are available in several configurations to provide a full range of options. Size select DNA in ranges between 100 bp up to 1.5 kb. Also, High-Pass protocols are available for collecting all larger DNA above a fragment size.

  • 3% agarose, 100 - 250 bp
  • 2% agarose, 100 - 600 bp
  • 1.5% agarose, 300 bp - 1.5 kb
  • 0.75% agarose, high-pass size selection

Workflow summary

The PippinHT instrument includes a monitor, keyboard, and mouse. A CPU is built in -> an external PC is not required.

Specifications

Electrophoresis voltage75V constant, 75V pulsed field
Optical detection470 nm excitation | 525 nm emission
Power requirements100-240 VAC, 2.5A, 50-60 Hz
Weight9 kg
Dimensions (W x D x H)33.0 x 64.0 x 46.0 cm

Applications

  • Whole genome sequencing
  • miRNA isolation
  • Paired-end sequencing
  • DNA sequencing
  • RNA-seq
  • Genotyping
  • ChIP-seq
  • Mate-pair

Resources

Cassette reference chart

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