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SensiFAST Probe Hi-ROX Kit

Reference: BIO82005

SensiFAST™ Probe Hi-ROX Kit has been developed for fast, highly reproducible real-time PCR and has been validated on commonly used real-time PCR instruments. A combination of the latest advances in buffer chemistry and PCR enhancers, together with a hot-start DNA polymerase, ensures that SensiFAST Probe Kit delivers fast, highly-specific and ultra-sensitive real-time PCR.

Features :

  • Highly specific - minimal non-specific activity leading to better efficiency and greater accuracy
  • Ultra-sensitive - perfect for difficult and low copy number samples
  • Efficient multiplexing - increasing throughput and conserving precious sample material
  • Fast - optimized for fast cycling conditions, ideal for high-throughput
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SensiFAST™ Probe Hi-ROX Kit has been designed for fast, highly reproducible real-time PCR and has been validated on commonly used real-time instruments. The kit is formulated for use with probe-detection technology, including TaqMan®, Scorpions® and molecular beacon probes. SensiFAST Probe Kit is optimized for fast mode on fast qPCR instruments and fast cycling conditions on standard qPCR instruments.

A combination of the latest advances in buffer chemistry and PCR enhancers, together with a hot-start DNA polymerase, ensure that SensiFAST Probe Kit delivers shorter run times with results that are highly reproducible, highly-specific and ultra-sensitive, making SensiFAST ideal for high-throughput assays. The advanced buffer chemistry and enhancers make SensiFAST Probe perfect for multiplexing.

For expression analysis, SensiFAST cDNA Synthesis Kit is the perfect partner for SensiFAST Probe Hi-ROX Kit for very fast cDNA synthesis and real-time results, without compromising sensitivity or quality.

SensiFAST Probe Hi-ROX is provided as a 2X mastermix containing all the components necessary for real-time PCR, including dNTPs, stabilizers and enhancers. SensiFAST Probe Hi-ROX contains premixed ROX for optional use.

Instrument Compatibility

ABI 7000, 7300, 7700, 7900, 7900HT, 7900HT FAST, StepOne™, StepOne™ Plus (see Specifications tab for product selection table) each of these instruments having the capacity to analyze the real-time PCR data with the passive reference signal either on or off, as well as several instruments that do not require the use of ROX.

  • Biomarker discovery and validation
  • ChIP
  • Drug therapy efficacy
  • Gene knockdown validation
  • Microarray validation
  • Pathogen detection
  • Cancer risk assessment
  • Detection of extremely low copy targets
  • Gene dosage determination
  • Genotyping, allelic discrimination, SNP, haplotyping
  • Microbial quantification
  • DNA damage measurement
  • Gene expression analysis
  • High‑throughput qPCR
  • Mitochondrial DNA studies

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