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RANGER Mix (Long Range PCR)

Reference: BIO25052

RANGER Mix is a ready-to-use 2X hot-start mix, designed for the amplification of long templates. The advanced formulation of RANGER Mix enables extreme sensitivity and increased fidelity. For convenience, all of the components necessary for trouble-free PCR reactions are supplied in one tube to reduce the number of pipetting steps and improve efficiency and reproducibility.

Features :

  • Efficient – specifically developed to give reliable amplification of fragments between 10 kb and 25 kb in length
  • Specific – incorporates an antibody-mediated hot-start enzyme
  • Fast – rapid enzyme activation and highly efficient amplification support reduced time to results
  • Flexible – ideal for amplifying a broad range of large fragments from even complex targets
  • Accurate – possesses 3’ – 5’ proofreading exonuclease activity

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RANGER DNA Polymerase is recommended for all Long PCR applications RANGER is an easy-to-use, high performance enzyme, specifically developed to amplify fragments up to 25 kb in length. RANGER contains a unique combination of a high-performance DNA polymerase and novel buffer system that deliver the improved efficiency necessary for reliable amplification of longer amplicons.

RANGER is an antibody-mediated hot-start enzyme that eliminates non-specific amplification during reaction set-up. The inactivated enzyme does not possess polymerase activity, thereby preventing the non-specific amplification, such as primer-dimer formation, that often hinder Long PCR reactions from the start.

RANGER Mix includes specially formulated buffering system containing dNTPs, MgCl2 and enhancers at optimal concentrations, designed to minimize the requirements for PCR optimization, in turn reducing time to results and eliminating the cost of unnecessary repeats. Furthermore, RANGER dramatically reduces the time needed to set up reactions, thereby minimizing the risk of contamination. Greater reproducibility is ensured by the reduction in the number of pipetting steps that can lead to pipetting errors.

  • Validated for human genomic DNA up to 25 kb
  • Suitable for TA cloning
  • Higher fidelity PCR amplification
  • Mapping of chromosomal translocation breakpoints
  • Structural variations studies

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