Precellys Evolution

BER100EV

Precellys Evolution is the universal tissue homogenizer gathering high flexibility and high efficiency for either routine or R&D projects. Especially designed for L3 laboratories, the Precellys Evolution is based on 10 years of sample preparation expertise.

Features :

  • Universal tissue homogenizer
  • High flexibility and high efficiency
  • Capability to process 4 different sizes of tubes : 0.3 ml in well-plate format, 0.5 ml, 2 ml, 7 ml and 15 ml
  • 30+ different lysing kits available
  • Offers optimal robustness, safety, throughput

More details

Description
Applications
Specifications
Related products
Resources

Description

Concept

The Precellys Evolution possesses the unique automatic tube locking system, a real push-and-play system. Its flexibility and high efficiency gives users the ability to grind, homogenize or lyse any type of soft or hard sample from animal, human, plant or microorganism in seconds.

Technology

The Precellys technology is based on two main features :

1- The 8-figure 3D-motion (multi-directional) movement of the tubes. The same high level of energy is given to the beads inside each tube providing the same homogenization efficiency to all the processed samples (up to 24 at once). Thanks to this complex movement, the Precellys Evolution can disrupt any type of sample (even the hardest ones) in seconds with a perfect reproducibility and with a limited heat generation.

2- The patented vacuum tube locking system during the process. The press’n block system helps the user to save time and energy. It facilitates the handling of the equipment and secures the tubes before the start of the grinding process.

Efficiency on every sample

Up to 10,000 rpm allowing to process and standardize any sample preparation in seconds. Even the hardest ones like hair, teeth, bones, can be fully grinded in a short period of time.

Easy-handling

The innovative and patented automatic tube locking system allows an easy handling of the Precellys Evolution by anyone.

Best life time

The high quality manufacturing of the Precellys Evolution drastically reduces the need of maintenance and additional cost over the years – The Precellys Evolution is made in France.

Adapted to any type of sample

The high flexibility of the Precellys Evolution, capable of handling 4 different tube sizes (0.5 ml, 2.0 ml, 7.0 ml and 15 ml), makes it the perfect tool when processing different sample types in size, weight or volume.

Protection for thermo-sensitive molecules

The Precellys Evolution is compatible with the cooling unit Cryolys. This combination allows to protect sensitive molecules when processing the samples.

Lysing Kits

Specifications

CapacityFrom 8 to 96 x 300 µL
From 1 to 24 x 2 ml / 0.5 ml
From 1 to 12 x 7 ml
From 1 to 6 x 15 ml
Speed rangeFrom 4,500 rpm to 10,000 rpm (increment by 100 rpm)
Locking systemAutomatic tubes locking system – patented
ProgramsThe software allows to save an unlimited number of protocols
Number of cyclesUp to 10
Cycles timeUp to 90 sec (increment by 1 sec)
Noise<70 dB
Power requirement90 – 250 VAC / 50-60 Hz, Power : 1 kVA
Dimensions (W x D x H)51.5 x 37.8 x 40.0 cm / 63.2 cm with opened lid
Weight27 kg

Applications

Lysing Kits

Cancer research

Hard tissues such as those derived from tumors can be particularly difficult to homogenize, and many cancer researches utilize animal models. Precellys homogenizer is perfectly adapted to such applications.

Most soft tissue types such as liver, spleen or pancreas are relatively easy to homogenize. However, hard tissues such as those derived from tumors can be particularly difficult to homogenize, and many cancer researchers utilize animal models such as mouse, rat, etc., to study cancer progression. Complete homogenization is needed to extract high quality DNA, RNA or protein for further downstream processing. For applications such as microarray or sequencing analysis, it is important to maintain reproducibility amongst biological replicates during nucleic acid extraction.

Agronomy

Agronomy researches gathering GMO analysis, food allergies and plant studies, often require to grind hard samples such as seeds, roots or frozen food which are particularly hard to grind.

Most soft samples types such as leaves are relatively easy to homogenize. However, hard samples such as seeds, roots or frozen food can be particularly difficult to grind. Complete grinding in dry or liquid conditions is needed to extract high quality DNA, RNA or protein for further downstream processing. For applications such as microarray or sequencing analysis, it is important to maintain reproducibility amongst biological replicates during nucleic acid extraction. It is particularly interesting to identify species, GMO and understand their development mechanisms. Moreover, allergen studies can be performed more efficiently thanks to the capacity to grind in dry condition.

Forensic

Extremely hard samples such as bone, teeth, and hair are common in forensic laboratories. Popular downstream applications include DNA extraction from bone or teeth and drug extraction from hair.

Extremely hard samples such as bone, teeth, and hair are common in forensic laboratories, and downstream applications include DNA extraction from bone or teeth and drug extraction from hair. Biological evidence is available in small quantities, and it is a challenge to extract high quality DNA from scarce, organic material.

In patients suffering from alcohol abuse, monitoring abstinence while undergoing alcohol withdrawal treatments is followed closely by physicians. Precellys homogenizer is used to extract ethylglucuronide (EtG), a highly specific alcohol biomarker, from hair samples using liquid chromatography tandem mass spectrometry. Precellys can be used to dry grind hard samples such as bone or hair, which typically represents a challenge for many forensics laboratories, and offers an alternative solution to improving DNA and drug extraction yields from scarce biological material.

Pharmacology

Pharmaceutical research starts with molecule leads identifications either from natural model or artificial.
In both cases, the DMPK or animal toxicology models analysis, require to homogenize tissues.

In preclinical dermatological research for instance, complete skin homogenization is critical to determine the amount of compound delivered into the dermis and epidermis, which helps select for the best formulation prototype. The mass spectrometry analysis is often used for such application.

In addition to the capabilities of the Precellys to homogenize animal tissue, these instruments can also be used for micro or nano-milling of poorly water-soluble drugs to enhance bioavailability in pharmacokinetic studies. In clinical formulation development, the amount of active pharmaceutical ingredient (API) is often limited, and co-micromilling or nano-milling with various pharmaceutical excipients has been tested on the Precellys to increase solubility and dissolution rate of poorly water-soluble drug compounds.

Environment

Soil and environmental analysis often focus on microbiology studies and pollution impact on wildlife.
Microorganisms included in a biological matrix are particularly challenging to lyse.

Lysing microorganisms that are included in biological matrixes such as leaves can be particularly difficult to homogenize, and many environmental analysis utilize plant models to study microbial expansion and its potential impacts on wildlife. Complete lysing is needed to extract high quality DNA, RNA or protein for further downstream processing. For applications such as microarray or sequencing analysis, Precellys homogenizers help to maintain reproducibility amongst biological replicates during nucleic acid extraction.

Microbiology

Microorganisms studies are critical as they are used for bio-production or health researches. They are difficult to lyse due to their thick cell wall, which makes nucleic acid extraction difficult.

Microorganisms are difficult to lyse due to their extremely small size and their thick cell wall which makes nucleic acid extraction complicated. Precellys equipment and adapted lysing kits containing small (< 1 mm) glass beads to offer a real rapid and effective alternative to usual methods as chemical lysis or manual grinding. As Precellys solutions were initially designed for BSE diagnosis, it was mandatory to be able to work in BSL3 laboratories. This unique feature allow the Precellys to be used for infective and virulent microorganisms studies. Today, Precellys 24 or Precellys Evolution can be used in BSL4 laboratories, the ones working on HIV or Ebola.

Resources