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SensiFAST Probe No-ROX Kit

BIO86005

SensiFAST™ Probe No-ROX Kit has been developed for fast, highly reproducible real-time PCR and has been validated on commonly used real-time PCR instruments. A combination of the latest advances in buffer chemistry and PCR enhancers, together with a hot-start DNA polymerase, ensures that SensiFAST Probe Kit delivers fast, highly-specific and ultra-sensitive real-time PCR.

Features :

  • Highly specific - minimal non-specific activity leading to better efficiency and greater accuracy
  • Ultra-sensitive - perfect for difficult and low copy number samples
  • Efficient multiplexing - increasing throughput and conserving precious sample material
  • Fast - optimized for fast cycling conditions, ideal for high-throughput

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Description
Applications
Specifications
Related products
Resources

Description

SensiFAST™ Probe No-ROX Kit has been designed for fast, highly reproducible real-time PCR and has been validated on commonly used real-time instruments. The kit has been formulated for use with probe-detection technology, including TaqMan®, Scorpions® and molecular beacon probes.

SensiFAST Probe Kit has been optimized for fast mode on fast qPCR instruments and fast cycling conditions on standard qPCR instruments. A combination of the latest advances in buffer chemistry and PCR enhancers, together with a hot-start DNA polymerase, ensures that the SensiFAST Probe Kit delivers shorter run times. It is also is highly reproducible, highly-specific and ultra-sensitive, allowing more samples to be run with the highest confidence, ideal for high-throughput assays.

The advanced buffer chemistry and enhancers also make SensiFAST Probe very sensitive and perfect for multiplexing. SensiFAST Probe No-ROX is provided as a 2X mastermix containing all the components necessary for real-time PCR, including dNTPs, stabilizers and enhancers.

For expression analysis, SensiFAST cDNA Synthesis Kit is the perfect partner for SensiFAST Probe No-ROX Kit for very fast cDNA synthesis and real-time results, without compromising sensitivity or quality. SensiFAST Probe No-ROX is used with real-time PCR instruments that do not require a passive reference.

Instrument Compatibility

Roche LightCycler® 480, LightCycler® Nano, Bio-Rad iCycler®, iQ®5, MyiQ™, Opticon™, Opticon™2, Chromo4™, MiniOpticon™, CFX96™, CFX384™, Cepheid SmartCycler®, Qiagen Rotor-Gene™ 3000, Rotor-Gene™ 6000, Rotor-Gene™ Q, Eppendorf Mastercycler®ep realplex, PCRmax Eco™, Takara Thermal Cycler Dice® (TP800), Analytik Jena qTOWER 2 and Techne Quantica®, PrimeQ. See Specifications tab for product selection table.

Specifications

Applications

  • Biomarker discovery and validation
  • ChIP
  • Drug therapy efficacy
  • Gene knockdown validation
  • Microarray validation
  • Pathogen detection
  • Cancer risk assessment
  • Detection of extremely low copy targets
  • Gene dosage determination
  • Genotyping, allelic discrimination, SNP, haplotyping
  • Microbial quantification
  • DNA damage measurement
  • Gene expression analysis
  • High‑throughput qPCR
  • Mitochondrial DNA studies

Resources

Product Guide

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