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microCLEAN PCR/DNA Cleanup

2MCL5

microCLEAN PCR purification provides a simple spin protocol that is quicker, cheaper and more flexible than other products. It offers excellent recovery, is reliable and can be used for both tubes and plates. Just add an equal volume of microCLEAN to the sample, follow the 1½ spin protocol and get clean PCR fragments/DNA in less than 15 minutes.

Features :

  • Rapid - column-free PCR clean-up
  • Efficient - post-PCR recovery up to 98%
  • Optional pink dye - for improved visibility and minimal pellet loss
  • Pure - isolated products are suitable for downstream applications

More details

Description
Applications
Resources

Description

microCLEAN is an extremely rapid and simple, one tube, 1½ spin spin DNA cleanup reagent. It can be used to clean up or concentrate any type of double stranded DNA, efficiently removing reaction buffers, enzymes, primers, primer dimers or dNTPs (labeled or unlabeled). It is therefore ideally suited to clean up PCR fragments, labeled probes, sequencing reactions etc.

microCLEAN uses an easy, no mess protocol, it requires NO additional manipulation, i.e. no phenol extractions, ethanol precipitations, glass milk or spin columns are required and the protocol takes less than 15 minutes to complete.

Compared to most other reagents and techniques, microCLEAN is very versatile. There is no limit to the size of the target DNA to be purified by this method and the protocol can be adapted for any sample size. After microCLEAN, the DNA can be resuspended in any desired volume. microCLEAN offers close to 100% recovery of clean DNA for further processing.

Applications

  • PCR fragments cleanup
  • Labeled probes cleanup
  • Sequencing reactions cleanup

Resources