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CleanDTR Big Dye Terminator Cleanup Kit

Reference: CDTR0005

CleanDTR is an efficient paramagnetic bead-based system, designed to remove unincorporated dye terminators from Sanger sequencing reactions.

Features :

  • Long Phred 20 read lengths averaging over 800 bps
  • Pass rates over 85% or higher
  • Efficient elimination of sequencing reaction contaminants
  • Reduce BigDye* usage, due to increased average signal strenght

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The CleanDTR process involves three simple steps including bind, wash and elute. While binding the sequencing product selectively to the magnetic particles, unincorporated dyes, nucleotides, salts and primers will be removed during ethanol washes. This principle allows for elution of the pure Sanger Sequencing product in the elution buffer of choice. The protocol can be adapted to your current liquid handling workstation (e.g. Beckman, Hamilton, Tecan, Caliper, Perkin Elmer, Agilent and Eppendorf) utilizing your current protocol as well as it can be performed manually.

Cleanup of sequencing product for both ABI and MegaBACE platforms

Supported Chemistries

  • BigDye* versions 1.0, 1.1, 2.0, 3.0 and 3.1
  • DYEnamic ET
* BigDye is a registered trademark of Applied Biosystems
Product numberDescription
Number of reactions
Storage conditions
CDTR0005 CleanDTR – 5 ml 500 * 4-8⁰C
DO NOT FREEZE
CDTR0050 CleanPDTR – 50 ml 5,000 *
CDTR0500 CleanDTR – 500 ml 50,000 *
* Number of reactions is based on a typical 10 μl PCR reaction volume.
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