The Clean Total RNA Kit allows for isolation of high-quality total cellular RNA from a wide variety of tissue samples and cultured cells. Total RNA can be isolated from 5-10 mg tissue or 1 x 106 cultured cells.
This product is no longer in stock
Samples are lysed using the CTR Lysis Buffer combined with Proteinase K. During lysis, proteinase K will remove proteins from the samples by digestion. With addition of ethanol and CleanNA CTR particles both DNA and RNA are bound. After 2 wash steps, the DNA and RNA will be eluted from the CleanNA CTR Particles. Genomic DNA is then removed with a DNase I digestion step after which the RNA will be rebound to the CleanNA CTR Particles. After two quick wash steps, RNA is eluted.
The isolated RNA is directly suitable for use in downstream applications such as RT-PCR, restriction digestion, and hybridization applications. Since the Clean Total RNA Kit uses CleanNA’s paramagnetic beads, it can also be adapted to most common automated liquid handling instruments.