Mic Real-Time PCR System

Speed

Mic uses a patented magnetic induction technology to achieve heating and forced airflow for cooling. This means faster heating and cooling times. Back that up with a robust optical system that reads all four channels simultaneously and running multichannel assays has never been quicker.

• Very fast cycling times with 35 cycles possible in less than 25 minutes*
• Instruments are available with either 2 (Green/Yellow) or 4 channels (Green/Yellow/Orange/Red)
• Separate high intensity LED excitation source for each channel (uniform excitation output) and a separate emission filter and detector for each channel

Accuracy

Mic’s spinning aluminium rotor gives unsurpassed temperature uniformity during dynamic and static operations. All other block-based cyclers only promise static uniformity, which could lead to inaccurate data, as samples aren’t heated and cooled equally. You might think this level of accuracy requires constant calibration, but Mic’s good to go right out of the box. You don’t need to calibrate – ever.

• Excellent reproducibility between samples and repeatability between runs and instruments ensures the highest level of quantification precision; allowing for the detection of two-fold differences in gene expression levels.
• Fixed optical path with no moving parts means no optical alignment or calibration and no need for a ROX reference dye.

Size

Mic takes up less space on the bench than your lab book. And weighing in at just 2 kg, this is the most portable qPCR cycler on the market. Even four Mic’s stacked together take up less bench space than your current cycler.

• The magnetic induction technology allows for a small instrument footprint to be achieved, by way of its elegant simplicity.
• The tube format uses 0.1 ml strips of four tubes and matching caps, supporting volumes of 10 to 25 µl. A tab on tube 1 ensures correct orientation of tubes into the instrument.
• High speed centrifugation ensures samples spin down at the start of a run, and during a run will remove bubbles and prevent condensation, eliminating the need for bulky heated lids and external centrifuges.

Connectivity

Multiple Mics can be operated from one workstation so 48 becomes 96, and 96 becomes 192. Bluetooth technology means fewer cables too.

• Instruments can communicate via Bluetooth or USB cable. As many as ten instruments can be run from the one PC allowing for up to 480 samples to be run simultaneously.
• Individual runs can be collated into a project and analysed together.
• Installation is plug-and-play.

Software

Next generation qPCR analysis software that is user-friendly and packed with intelligent features. See your results with detailed statistical analysis as soon as your run has completed. Designed to meet MIQE specifications, the Mic software offers you the most up-to-date qPCR analysis.

Projects

Take advantage of Mic’s modular functionality and amazing reproducibility by combining multiple runs from multiple instruments into one analysis. With the ability to combine up to 10 runs you can analyse up to 480 samples at one time. Now there is no need to wait for samples to be batched into one 96 or 384 well run. Complete the runs at the time you need them done and see the results now, not later.

Quantitation

Confidently detect 2 fold differences in gene expression levels. Whether it’s using standard curves for Absolute Quantification or determining gene expression using Relative Quantification through REST, know that the performance you get from the Mic will always ensure the highest level of quantitative precision.

Extreme quantitative precision

Detect differences within one cycle. When you need to quantify small differences in relative gene expression, Mic will deliver the extreme levels of quantitative precision you need. Especially for bacterial genetics where minor differences in gene expression can multiply into big differences.

A wide linear dynamic range

Down to single digit copies of DNA. Have the power to detect high and low copy numbers of your target. Be it using Absolute Quantification to detect viral loads, or simply determining a PCR efficiency using your standard curve, Mic will deliver the dynamic range you need, accurately and precisely.

Outstanding reproducibility

Get the same result across multiple runs and instruments. We build our instruments to perfection so that each one is identical to the next. This means we can reproduce the same result not just across multi-runs, but instruments as well. Now you can combine multiple runs with minimal concern for variation. Inter-run calibrators become more of a quality control measure than a correction tool. And using sophisticated analysis software incorporating intelligent methods such as LinRegPCR, means we can further improve the qPCR reproducibility by minimising human error through analytical automation.

Fast cycling

Maintain assay performance even at speed. Get high quality data, fast ! Mic’s speed is on par with the fastest instruments on the market. But unlike the competition Mic’s superior temperature uniformity and accuracy means you don’t sacrifice on the quality of your qPCR. Completing runs in under 30 minutes is the new standard with Mic, not the exception.

Four colours

Lowest possible cross talk. If it’s molecular diagnostic (MDx) detection of pathogens, or genotyping using Allelic Discrimination, Mic has highly optimized filter sets to minimize your dye cross talk when multiplexing your real time PCR. With dedicated high powered LED and detectors per channel, detect all four colours in 1 sec during acquisition. No dye colour compensation or dye calibration needed – ever !