Myra Pipetting Robot

Simplicity

So effortless you won’t even notice it. A perfectly seamless workflow between the Myra liquid handling system and Mic cycler for qPCR. Run Myra and Mic instruments from the same one user interface. No exporting or importing of sample names required. Just setup, run, and analyse all in one location. Simplified solutions for standard laboratory processes including qPCR, NGS library quantification and more, all available at the click of a button. Setup experiments for multiple Mic cyclers using one Myra liquid handling system and analyse together in one file using the Project software feature. Calibrate Myra at the click of a button. No more sticking your head under the hood and eye balling the positions. Myra does it all for you.

Strong points

A robot with a vision to perfection !

• Best in class accuracy and precision of less than 10% for 1 ul pipetting volumes
• High precision pipette tip positioning for small aperture tubes such as 384 well plates
• Pressure based liquid level sensing that can monitor the aspirate and dispense process for errors
• The first liquid handling system with an integrated camera and state of the art algorithms to enable Myra to see what it needs to do. Myra automates and simplifies pipette calibration and monitors for user errors in deck layout such as missing tubes*
• An interchangeable pipette head gives you more flexibility with your workflow. Each pipette head contains its own calibration data removing the need to re-calibrate on each exchange
• Multi-dispense pipetting with greater accuracy to reduce time and save on pipette tips
• Using 384 well pipette tips means you swap out tips less often

Key features

Size

A dynamic design with style and function. At less than 10 kg and a footprint of less than 1,700 cm², Myra is half the size of other in class liquid handling systems. A closed pipette tip waste container minimises the footprint area and reduces contamination risk. UV LED lights and a compact HEPA filter reduce the chances of qPCR contamination. The compact design of both parts reduces size and weight of the Myra unit.

Deck space

Fewer SBS positions does not mean more restrictions. In fact, through clever flexibility in how we use each position we have created a small robot with more capabilities than robots twice the size. Myra’s software allows you to use any position on a plate or block as a sample, reagent, mix or standard. The smart design Myra multipurpose block provides flexibility for numerous tube types. Tube adaptor inserts enable the use of tubes as small of 0.1 ml up to as big as 10 ml bottles. And the 384-well rack tips frees up the deck space for your plates.

Tips

Reduce the number of times you need to swap out tips with the 384-well rack 50 µl tips. This also helps reduce the space on the deck to allow more space for other plates. These tips improve accuracy and precision for smaller volumes and reduce tip retention. They also afford better position precision for smaller tube openings.

Pressure level sensing

Myra uses pressure sensing to detect the liquid level. This reduces the risk of detecting bubbles and being affected by static. There’s also the convenient truth of not having to pay for expensive conductive tips. Further, the head can detect the level of aspirate and dispense giving more accurate feedback on the pipetting during a run.

Software

A dynamic design with style and function. From library quantification to normalised library pooling. Unlike any other for a liquid handling system. Configure input samples and output reactions, layout your deck and then start your run. So easy. Set it up yourself or let Myra figure it out for you. With a custom mode for creating your own setup to a fully automated setup using the intelligent option, we’ve got you covered.

There are inbuilt options for common applications such as qPCR and NGS library quantification all fully integrated with the Mic qPCR cycler for a seamless workflow. Applications such as NGS library preparation are also be available. Select the option in the start menu and follow the easy steps to getting the run started.

Myra advantages for normalisation and pooling

The Myra liquid handling system is highly regarded for its precision and reliability in setting up qPCR for NGS library quantification. Compared to other platforms, Myra produces superior results, making it a preferred choice for normalization and pooling tasks. One of the key advantages of using Myra is its level sensing feature, which can detect air pockets in wells-a common challenge that other platforms often struggle with. This capability ensures that each sample is pooled accurately, minimizing the risk of sample drop-outs.

Setting up normalisation and pooling on using the Workbench software is a simple as four easy steps:

• Import of enter your sample names and concentrations
• Select the concentration to normalise samples to. The software will calculate a maximum value for you
• Choose the number of samples per pool
• Enter the volume of each sample or total for the pool

The software will calculate the rest for you. It also has smart features to help determine how to handle samples that might be too high or too low to be normalised. Say good bye to having to calculate in Excel.

Deck layout

Laying out your deck takes no time or effort. Select the plates you need or use a predefined default. Move your reagent components, intermediate mixes, and templates over to the plates and you’re done. Almost all plate positions can be used as a template, mix, reagent or reaction. This level of flexibility opens up a lot more room on your deck. We cater for most generic tube types so finding the right plate won’t be a problem.

You don’t find your tube type ? No problem – the software lets you define your own. How nice is that.

Case study: Illumina DNA Prep Kit

24 samples validation using the Illumina DNA Prep kit. Libraries displayed low variability across replicates, even barcode balance, and high coverage of sequence alignments.

Experimental setup

To demonstrate capability, 8-sample and 24-sample plates of 150 ng of input DNA (48 kB genome Lambda DNA and Human Genomic DNA) were prepared on the Myra Liquid Handling System. Manual preparation of an 8-sample plate of 150 ng of input DNA (48 kB genome Lambda DNA) was also performed as a comparison method. The quality of these libraries were checked with BiOptic Qsep1-Plus bio-fragment analyser (Figure 1). The libraries were quantified using NEBNext Library Quant Kit for Illumina with reaction setup on the Myra Liquid Handling System and amplification on the Mic qPCR Cycler. Libraries were simultaneously normalised and pooled to 2 M using the Myra Liquid Handling System, then sequenced on a 2×150 bp flow cell on an iSeq 100 System for whole genome sequencing. Q30 and percentage PF were used to show the quality of the libraries. Sequencing data was demultiplexed using Illumina’s BaseSpace Sequence Hub and coverage maps of the Lambda genome were analysed using Geneious Prime 2022.2.2.

Figure 1. Capillary electrophoresis gel of library quality analysed on BiOptic Qsep1-Plus with S2 Cartridge for 24-plax Lambda libraries. Consistent size and intensity was achieved for all 24 libraries indicating good library prep on the Myra liquid handling system.

Results

We were able to successfully generate high-quality 8-plex and 24-plex DNA libraries on the Myra, with comparative performance to the manual method (Figure 2). Fragment analyzer results show consistent fragment sizes across all 24 DNA libraries (Figure 1). The quality of the sequencing runs was comparable with a Q30 score > 90% for all methods. Barcode balance was also accurate, with variability less than the manual method. Sequencing coverage of the Lambda genome showed consistent uniformity across 8-plex and 24-plex DNA prep libraries constructed on the Myra. The sequence data showed low sample variability and reliable uniformity for libraries prepared using Illumina DNA Prep, reinforcing the benefits of using an automated NGS library prep workstation..

Figure 2. Mean library yield for DNA processed on Myra vs. manual preparation.

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Calibration

Eye can do it. With a world first integrated high precision camera, achieving calibration on a liquid handling system has never been this easy. Simply click to the middle of the tube image, and let the software do the rest.

Only calibrate what you need. Why spend all day calibrating tubes you won’t even use. The Myra software will only require calibration of tubes that are in use. All calibration is recorded to the robot so no need to worry about doing it all again if you change computers or need to exchange the pipette head.

Performance

The key to excellent data starts with Accuracy and Precision. Accuracy is the ability to achieve the right value. Precision is the ability to reproduce the same value. For automated qPCR setup, you need to be able to do both – really well. The Myra pipetting robot can. The proof is in the results.

Precision

Means sample repeatability. With an automated pipetting precision of < 10% CV at 1 µl, the Myra robotic pipette will enhance your qPCR repeatability. Whether it’s for your gene expression studies or HRM genotyping, you will be confident in the data when setting up on the Myra pipetting robot.

Figure 3. KRAS human genomic DNA target. 10,000 copies per reaction. 48 replicates setup using 1 µl of template per 25 µl reaction.
C_q = 19.23 | σ = 0.05 | Δ_max-min= 0.25 | CV = 0.3%

Repeatability

Even at low copy number. Maintain confidence in your data even when you are working with samples that are influenced by the unavoidable Poisson distribution. The Myra automated qPCR setup ensures that the only significant bias in your experiments comes from the probability of aspirating the template and not the performance of the automated pipettor.

Precise every time

Reproducible results to ensure experimental integrity. We know that the most important part of any scientific work is being able to reproduce your data. With the Myra liquid handler and Mic real-time PCR instrument, that’s exactly what we aim for. Achieve equivalent experimental setup each time, every time. Even when working with very low copy numbers.

Accuracy and precision maintained

Even at the extreme ! Push the limits of your automated qPCR. A 1.2 fold dilution series is now the norm, not the exception when using the Myra automated liquid handling system. With Myra’s amazing pipetting accuracy and precision combined with Mic’s incredible temperature uniformity ; uncommon has become routine.

The art of accuracy and precision

Be creative with your lab automation. With a robotic liquid handler capable of high pipetting accuracy and precision – we though we would be a little creative. So we decided to pipette a complex patterned dilution series to add a touch of flair to our already good looking robotic pipette. The result speaks for itself.

*coming soon