Sage Science is pleased to introduce the Pippin Pulse Electrophoresis System for analytical gels. The system resolves DNA to 100 kb and beyond, economically, and without unwieldy instrumentation and/or chillers. Pulsed-field protocols are programmed and run from a PC, providing users the flexibility to develop customized protocols to meet specific applications. The operating range is 25 - 175V, and up to 200 mA.
The Pippin Pulse Electrophoresis System includes the power supply, gel box, cables, software (for PC) for programming, and user manual. A netbook can also be delivered, optionnally.
About Pulsed (Field Inversion) Electrophoresis
Pulsed-field gels work by shuttling DNA back and forth in the gel, effectively slowing down large DNA fragments that might otherwise run at the same rate as smaller ones. By switching the direction of the electric field in a gel, DNA will change its direction of migration. Since smaller molecules can change direction faster than larger molecules, more differentiated separation can be achieved by rapidly switching, or pulsing, the electric field. For resolving a wide range of fragment sizes, the length of the forward and reverse can be incrementally increased or decreased, and cycled.