SensiFAST SYBR® No-ROX Kit has been developed for fast, highly reproducible real-time PCR and has been validated on commonly used real-time instruments.
The latest advances in buffer chemistry and enhancers, together with an antibody-mediated hot-start DNA polymerase system, ensure that the SensiFAST SYBR® No-ROX Kit produces fast, highly-specific and ultra-sensitive real-time PCR.
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The antibody-mediated hot-start DNA polymerase system reduces the chances of primer/dimers formation. This allows a much greater dynamic range by removing competition for reaction components during amplification, in turn allowing greater sensitivity. The addition of the latest advances in buffer chemistry and enhancers also ensures that the SensiFAST SYBR® No-ROX Kit produces fast, highly-specific and reproducible real-time PCR results in under 30 minutes. For expression analysis, SensiFAST cDNA Synthesis Kit is the perfect partner for SensiFAST SYBR® No-ROX Kit for very fast cDNA synthesis and real-time results, without compromising sensitivity or quality.
For added convenience, SensiFAST SYBR® No-ROX is provided as a 2X mastermix containing all the components necessary for real-time PCR, including the SYBR® Green I dye, dNTPs, stabilizers and enhancers. SensiFAST SYBR® No-ROX is used with real-time PCR instruments that do not require a passive reference.
Roche LightCycler® 480 platform, LightCycler® Nano, Bio-Rad Opticon™, Opticon™2, Chromo4™, MiniOpticon™, CFX96™, CFX384™, Cepheid SmartCycler®, Qiagen Rotor-Gene™ 3000, Rotor-Gene™ 6000, Rotor-Gene™ Q, Eppendorf Mastercycler®ep realplex, PCRmax Eco™, Takara Thermal Cycler Dice® (TP800), Analytik Jena qTOWER and Techne Quantica®, PrimeQ.