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SensiFAST Probe No-ROX One-Step Kit

Reference: BIO76005

SensiFAST™ Probe No-ROX One-Step Kit has been developed for fast, highly reproducible real-time PCR and has been validated on commonly used real-time PCR instruments. A combination of the latest advances in buffer chemistry and PCR enhancers, together with a hot-start DNA polymerase and  unbiased cDNA synthesis, ensures that SensiFAST Probe One-Step Kit delivers fast, highly-specific and ultra-sensitive real-time PCR.

Features :

  • Sensitive – optimized buffer formulation delivers reliable quantification from even very low copy number RNA targets
  • Reproducible – consistent results between technical replicates for increased confidence in results
  • Robust – reliable detection of RNA targets from a broad range of sample types
  • Fast – delivers reproducible, accurate assay results in as little as 40 minutes
  • Efficient – excellent performance in multiplex assays

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CHF 892.00
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Packaging: 500 rxns (20 µl)
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The SensiFAST™ Probe No-ROX One-Step Kit has been optimized for fast, efficient, unbiased cDNA synthesis and subsequent highly-sensitive, reproducible real-time PCR detection in a single tube. SensiFAST Probe One-Step has been optimized to deliver excellent results in both singleplex and multiplex assays.

An antibody-mediated hot-start DNA polymerase promotes rapid activation and supports highly-specific amplification, which in turn improves assay sensitivity and dynamic range. A combination of the latest advances in buffer chemistry and PCR enhancers confer superior assay performance under fast thermal cycling conditions. The inclusion of separate RiboSafe Inhibitor ensures accuracy by protecting RNA targets from RNase degradation.

The SensiFAST™ Probe No-ROX One-Step Kit has been validated on all commonly-used real-time instruments that do not require the passive reference dye ROX. SensiFAST Probe One-Step has been formulated for use with dual-labelled probes, including TaqMan®, Scorpions® and molecular beacon probes.

Instrument Compatibility

Roche LightCycler® 480, LightCycler® Nano, Bio-Rad iCycler®, iQ®5, MyiQ™, Opticon™, Opticon™2, Chromo4™, MiniOpticon™, CFX96™, CFX384™, Cepheid SmartCycler®, Qiagen Rotor-Gene™ 3000, Rotor-Gene™ 6000, Rotor-Gene™ Q, Eppendorf Mastercycler®ep realplex, PCRmax Eco™, Takara Thermal Cycler Dice® (TP800), Analytik Jena qTOWER 2 and Techne Quantica®, PrimeQ. See Specifications tab for product selection table.

  • RNA viruses detection and quantification
  • Multiplexing
  • Gene expression
  • Absolute quantification
  • SNP detection
  • Cellular mRNA and miRNA
  • Drug therapy efficacy
  • Gene knockdown validation
  • ChIP
  • Cancer risk assessment
  • Cellular mRNA and miRNA

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