Blue Pippin PLUS Cassette 0.75% HMW, External Marker

The High Pass Plus™ gel cassette is the newest addition to the Pippin Family. As the name suggests, it is dedicated to our BluePippin “High-Pass” DNA size selection which has been a go-to method for increasing the read lengths for long-read sequencing.

High Pass size selection removes smaller DNA fragments from a sheared genomic DNA (or sequencing library) while collecting the remaining larger fragments above a tightly controlled size threshold. This way, larger molecules can be presented to the detector or droplet, and better sequencing performance can be achieved.

Long-read sequencing sample prep has been improving overall, in terms of set-up time and workflow. Sage Science decided to look at the High Pass as well to see if they could optimize the approach. To this end, they designed an entirely new gel cassette dedicated to High Pass– the High Pass Plus. Moreover they were able to cut the runtime in half and improve performance and yield.

Here’s a comparison between the standard BluePippin Cassette and the High Pass Plus:

The High Pass Plus cassette has a stocky separation column, so DNA has a shorter distance to travel. The wider column and increased taper provide higher resolution for a cleaner and more accurate size cut-off. The larger sample wells now allow a maximum load of 10 μg (100% up from standard Blue Pippin cassettes). Also the size of the elution module has been increased as well as the surface area of the filtration membrane, bringing about improved sample recovery and reproducibility.

Blue Pippin software requirement: v6.31/6.40 CD31