Blue Pippin

Targeted size selection for Next-Gen Sequencing (100 bp – 50 kb)

The BluePippin has the DNA capabilities of the Pippin Prep, plus the extra benefit of pulsed-field electrophoresis for resolving and collecting high molecular weight DNA.  With SDS-agarose gel cassettes, the BluePippin collects targeted protein sizes from complex samples for biomarker or protein modification studies. Target sizes or ranges of sizes are entered in software, and fractions are collected in buffer. Up to 5 samples/gel cassette may be run, with no possibility of cross contamination.

Pre-cast agarose gel cassettes for DNA size selection

Gel cassettes for DNA selections are available in four agarose concentrations to provide a full range of size selection options between 100 bp up to 50 kb.  For smaller fragment sizes, internal standards may be used for higher accuracy and increased sample capacity (from 4 to 5 samples/cassette).  Higher molecular weight fragments require a dedicated lane to run a calibration marker. Run times are as little as 30 minutes; to resolve very large DNA fragments, pulsed-field runs may last several hours. View the BluePippin cassette reference chart to explore the DNA size selection options and run times.

• 3% agarose, 100 - 250 bp
• 2% agarose, 100 - 600 bp
• 1.5% agarose, 250 bp – 1.5 kb
• 0.75% agarose, 1 - 50 kb

Workflow summary

• Mate-pair sequencing
• Single molecule sequencing
• miRNA isolation
• DNA sequencing
• RNA-seq
• Genotyping
• ChIP-seq
• Protein mass spec