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CellDrop Cell Counter

Reference: CellDrop

CellDrop Cell Counters feature unique DirectPipette™ Technology to eliminate plastic slides and cumbersome hemocytometers from routine cell counting.

Features :

  • DirectPipette™ Technology
  • Accurate across the widest range of cell size and density
  • Easy-to-learn, powerful-to-use
  • Available in Dual Fluorescence and Brightfield (CellDrop FL) or Brightfield only (CellDrop BF) models

CellDrop Cell Counters feature unique DirectPipette™ Technology to eliminate plastic slides and cumbersome hemocytometers from routine cell counting. Featuring dual fluorescence and/or brightfield optics, variable height sample chamber, and powerful, easy-to-use analysis software, the CellDrop enables the fastest cell counts, viability assessment, and GFP transfection efficiency measurements across the widest range of cell density, cell type and application.

DirectPipette™ technology

The CellDrop Series brings the familiar Load, Measure & Wipe Clean functionality of microvolume spectrophotometers to cell counting. Traditional plastic slides or glass hemocytometers are replaced by two permanent optical sapphire surfaces. These are positioned parallel to one another by a calibration-free, high-precision mechanism, forming a chamber of precisely defined height. 10 µl of cell suspension is pipetted into the chamber, analyzed and simply wiped away with a dry laboratory wipe. The powerful live-view image allows instant verification that cleaning has been successful.

CellDrop_directpipette.jpg

Accurate across the widest range of cell size and density

Using conventional counting methods, samples with high or low cell densities require time consuming dilution or concentration steps. The variable height chamber of the CellDrop adjusts chamber depth automatically, delivering the most accurate counts over the widest cell densities – 7 x 102 to 2.5 x 107. Chamber height can also be adjusted to accommodate almost any cell with a diameter range of 4 to 400 µm.

Easy-to-learn, powerful-to-use

Designed by life scientists, the CellDrop EasyApps® Software exceeds the functionality of high cost cell counters while delivering the ease of use of simpler systems. EasyApps combines with a high definition, 7″ touchscreen interface to give error-free operation with sample loading to data exporting in seconds. Pre-installed with a range of common applications for one-touch analysis of assays such as Brightfield, Trypan blue, AO/PI, GFP and Yeast and the ability to create custom methods makes the CellDrop a flexible workhorse for the busy, multi-user lab.

Hazardous samples ?

The small footprint and onboard processing of CellDrop allow it to fit inside most flow hoods where required. For applications that require the cells to be contained when reading, all instruments also have the ability to read samples in common disposable plastic slides and reusable slides without the need for additional adaptors.


Models

Available in Dual Fluorescence and Brightfield (CellDrop FL) or Brightfield only (CellDrop BF) models with many features :

  •   Intuitive app icons for quick recognition
  •   7″ HD Touchscreen, fluid pinch and zoom, glove compatible touch interface
  •   Live HD preview of cell samples
  •   Real-time cell size gating on main results screen
  •   High definition cell outlining
  •   Password protected user accounts for project and data management
  •   Thousands of measurements stored onboard instrument (60 GB SSD is standard)

Pay-As-You-Go

Get all the convenience of consumable-free cell counting with a lower initial investment. The PAYG option is ideal for labs with smaller capital budgets or lower throughput. Simply purchase a bundle of Count Codes according to your lab needs and EasyApps will use one for each sample measured.

Apply codes to a single account or share amongst users. With the robust admin options available in EasyApps® you are in control and if your needs change, simply upgrade to the Unlimited (UNLTD) version of the CellDrop product.

The DeNovix CellDrop Series are stand-alone instruments with intuitive, easy-to-use apps for automatic cell counting and viability analysis :

CellDrop Menu.jpg

Brightfield apps (all models) :

  • Fast count of Brightfield cell image
  • Live/dead assessment using Trypan Blue assay
  • Cells/ml, cell size and % viability reported

Cell gating :

CellDrop_cellgating.jpg

Cell Size Histograms allow easy gating for non-target cells or cell debris. Simply drag the slider to set the cell size of interest.

Declustering :

cell%20gating.jpg

High Definition cell outlining and declustering algorithms developed by DeNovix provide high accuracy cell counts of regular and irregular cells even at high density.

Fluorescence apps (CellDrop FL only) :

  • All Brightfield capabilities plus 2 channel fluorescence (green and red)
  • Cell viability analysis using acridine orange (AO) and propidium iodide (PI)
  • Analyze primary cells in the presence of debris
  • AO for identification of nucleated cells
  • PI for cell viability
  • AO/PI for live/dead
  • Yeast viability
  • GFP transfection efficiency
  • 6 intuitive results analysis formats

Primary cells :

primary_cells.jpg

Viability measurements of PBMCs in the presence of debris and red blood cells. The unique combination of flexible hardware and custom software ensures compatibility with a broad range of cells and assays.

Dynamic range 7 x 102 to 2.5 x 107 cells/ml
Sample volume 3.3 µl (high density), 10 µl (standard), 40 µl (low density)
Measurement speed Brightfield -> 3 seconds
AO/PI -> 8.5 seconds
CellDrop FL modes Dual Fluorescence
Single Fluorescence
Brightfield
Fluorescence + Brightfield
CellDrop BF modes Brightfield only
Sample surfaces Optical Sapphire
Brightfield illumination LED 530 nm
Fluorescence illumination LED 470 nm
Emission filters AO 525 nm ± 25 nm
PI 645 nm ± 37 nm
Gesture recognition Multipoint touch, swipe, pinch
Display High Definition 1280 x 800 7” color display
Glove compatibility All common lab gloves
Camera FL Basler acA2040-55 µm
BF Basler daA2500-14 µm
Images FL 2048 x 1536 px (3.15 MP)
BF 2592 x 1944 px (5 MP)
with overlay capabilities
Focus Software controlled course and fine adjustment
Detector FL Sony IMX265
BF ON Semiconductor MT9P031
Processor NVIDIA Tegra K1 with Quadcore ARM Cortex-A15
Connectivity Wi-Fi, Ethernet, HDMI, 3 USB ports
Dimensions (W x D x L) 21.0 x 18.0 x 37.0 cm
Weight 8 kg
Operating voltage 12 VDC

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