GRGreen Nucleic Acid StainView larger

GRGreen Nucleic Acid Stain


GRGreen is a nucleic acid stain for detecting nucleic acids, e.g., double-stranded DNA, in agarose gel. It can be used for replacing mutagenic Ethidium Bromide (EB).

Features :

  • Available at 10,000X in H2O for better safety
  • Compatible with UV or blue light transilluminator and common gel documentation systems
  • Does not affect downstream experiments
  • Also compatible with sodium borate electrophoresis buffer
  • Cut out DNA bands for subcloning under safer blue light : no mutations caused by EB and UV light

More details

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Protocol :

  1. Prepare 20 to 40 ml of agarose gel solution (concentration from 0.7~2.0%) with TAE, TBE or Borate Buffer in a 250 ml flask and mix it thoroughly. Place the flask in the microwave, heat on high until the solution is completely clear and no small floating particles are visible (about 2~3 minutes).
  2. After the gel solution cools to about 55°C, add GRGreen to the solution to a 1X final concentration. Swirl the flask gently to mix the solution and avoid forming bubbles.
  3. Pour the gel solution into a gel tray until the comb teeth are immersed about 1/4~1/2 into the gel solution.
  4. After the agarose gel has solidified you can perform electrophoresis using either 0.5 to 1X TAE, TBE or borate buffer.
  5. Detect the bands using UV or blue light transilluminator.