BeST LAMP PCR Kit

LAMP (Loop Mediated Isothermal AMPlification) is an isothermal nucleic acid amplification technique. In contrast to polymerase chain reaction (PCR) technology, which consists of repeated steps carried out in different temperatures, isothermal amplification is performed at a constant temperature and does not require a thermal cycler.

Advantages of LAMP technique

It allows for efficient and highly specific amplification of DNA by using three pairs of primers complementary to a suitable sequence of targeting DNA. Due to the specificity of primers the quantity of amplified DNA in the reaction is also considerably higher than in the PCR.

Fast amplification of DNA enables quick and easy identification of human, animal and plant pathogens. The detection time (from 30 min to 1.5 hours) is dependent on the quality and concentration of the target DNA. In case of phage lambda DNA with a concentration range between 5–50 ng/μl the amplification time of the DNA sequence is less than 30 min.

BeST™ polymerase used in the LAMP reaction is a modified version of Bst polymerase from Bacillus stearothermophilus, lacking the 5’-3’ exonuclease domain. In addition to a replication activity BeST™ polymerase has high strand displacement activity which eliminates denaturation of the DNA template.

The amplified products are concatemers of different sizes forming a ladder-pattern structure which could be detected by agarose gel electrophoresis or by monitoring the amplification in real time using fluorescent dye binding to double-stranded DNA or dyes sensitive to pH changes.