DS-11 FX Spectrophotometer Fluorometer

DS11FX

The DS-11 FX Series instrument rapidly quantifies over seven orders of magnitude of nucleic acids and protein samples selecting your choice of UV-Vis or Fluorescent modes.

Features :

  • The only all-in-one absorbance and fluorescence design
  • Most sensitive 1 µl UV-Vis available
  • Broadest dynamic range
  • Stand-alone (no PC required)
  • Maintenance and calibration free

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Description
Applications
Specifications
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Description

Best-in-Class performance and features

Compare the DeNovix DS-11 Series vs. the NanoDrop™ One spectrophotometer and the Qubit® Fluorometer. Awarded 2017 and 2018 reviewers' choice product of the year and Platinum Seal of Quality 2018.

The only all-in-one absorbance and fluorescence design

Equip your lab for today’s quantification requirements and for future assay challenges. The choice of microvolume or cuvette absorbance and fluorescence methods delivers unmatched flexibility and dynamic range. Coupled with the DeNovix dsDNA quantification assays, a range of 0.5 pg/µl to 37,500 ng/µl can be rapidly measured.

Total measurement confidence

The patented* DS-11 FX combines unique hardware and software features to deliver unmatched confidence in sample accuracy, performance and reliability.

  • SmartPath® Technology  – real time, precise pathlength control delivered every time
  • Exceptional for proteins – BridgeTesting® eliminates broken sample columns common on other platforms
  • SmartQC™  Contamination Alerts – Instant sample QC guidance
  • Calibration and maintenance free. No measurement drift, no service down-time. Accurate results every time.

* Patent US9442009

Easy to learn. Fast to use.

DS-11 FX Series instruments feature the most powerful and intuitive software of its type. Designed by life scientists, pre-installed EasyApps® and a breakthrough Android™ touchscreen interface delivers error-free operation and sample to data in under 4 seconds. Every instrument is ready to measure right out of the box – no PC set-up or software installation.

Microvolume mode

SmartPath® Technology

SmartPath Technology is at the heart of all microvolume measurements on the DS-11 FX Series. The proprietary algorithms provide ultra-precise by using real-time absorbance feed assessment to determine the optimum pathlength with which to measure the sample. This enables a best-in-class lower detection limit (0.75 ng/ul dsDNA, 0.60 ng/ul RNA or 0.04 mg/ml BSA) and the highest max detection (37,500 ng/ul of dsDNA or 1,125 mg/ml of BSA protein).

BridgeTesting®

A major concern for microvolume spectrophotometers, especially those that use a 1 mm pathlength, is sample column breakage. When this occurs, the upper and lower measurement pedestals are not bridged with sample and the measurement will be erroneous by as much as 1,000% (not a typo). The DS-11 FX Series is inherently less susceptible to this since the max pathlength is 0.5 mm and the sample is continually compressed during measurements rather than stretched. In addition, DeNovix has developed a proprietary algorithm that detects in real-time if the sample column has broken and then compensates to ensure accurate results are achieved with no user action being required. No other microvolume spectrophotometer has this capability and this method is especially beneficial for quantification of low surface tension fluids such as protein samples. You can learn more about BridgeTesting technology in this TechNote.

SmartQC™ contamination alerts

DeNovix SmartQC™ software alerts the user and advises on potential issues.  Its features include :

  • Automatically identifies and flags samples with suspected contamination
  • On-screen guidance to potential sources of contamination
  • Advanced spectral analysis and alerts to sample measurement anomalies
  • Surface Check – alerts users if previous sample hasn’t been removed
  • Report data with quality control metrics

Always calibrated

Since the DS-11 FX Series does not rely on fixed path length calibrations and therefore does not require regular calibration checks. There is no downtime or expense required for routine calibration like many other spectrophotometers. DeNovix offers a LC-NA verification standard for labs that may need to verify proper operation from time to time in accordance with their own quality standards.

Truly 1.0 ul sample volumes

A key feature of the DS-11 FX is that the longest path length used (0.5 mm path) is shorter than the 1.0 mm path used by other microvolume spectrophotometers. This enables the DS-11 FX to truly measure 1.0 ul samples, virtually eliminating the possibility of erroneous results due to sample column breakage.

Fluorescence mode

Fluorescence freedom !

The most sensitive and precise fluorometer of its type provides the ability to run almost any common fluorescence assay. Choose from a wide range of pre-configured kits or create custom applications for total flexibility.  Combine the DS-11 FX with the DeNovix dsDNA fluorescence quantification kits for industry-leading sensitivity and dynamic range. Quantify to 0.5 pg/µl up to 4,000 ng/µl with your choice of three quantification kits.

Assay independance

Choose the assay you wish to work with, not the one dictated to you by a fluorometer manufacturer. DeNovix EasyApps® are pre-configured with a large selection of commercially available quantification assays. Choose easy two-point standard curve DeNovix Fluorescence Assays or Qubit® and Quantus™ assays for dsDNA, RNA or protein quantification.

Multiple channels with the researcher in mind

DeNovix Fluorometers include four emission and excitation channels to provide scientists the flexibility to use the fluorophore and detection ranges of their choice. Choose from UV (375 nm), Blue (470 nm), Green (525 nm) or Red (635 nm) excitation sources.

Assays included in DeNovix EasyApps®

Choose from one of these pre-programmed assays, or create your own !

  • Fluoro dsDNA : DeNovix dsDNA Broad Range, DeNovix dsDNA High Sensitivity, DeNovix dsDNA Ultra High Sensitivity, Quant-it™ PicoGreen® , Quant-it™  dsDNA HS, Quant-it™  dsDNA BR, Qubit®dsDNA BR, Qubit®dsDNA HS, Quantifluor® dsDNA and Quantifluor® One dsDNA
  • Fluoro RNA : Quant-it™ Ribogreen, Quant-it™ RNA BR, Quant-it™ RNA HS, Qubit® RNA BR, Qubit® RNA HS, Quantifluor® RNA, Quant-it™ microRNA, Qubit® MicroRNA
  • Fluoro ssDNA : Quant-it™ OliGreen, Qubit® ssDNA, Quantifluor® ssDNA
  • Fluoro Protein : Quant-it™ Protein, Qubit® Protein

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Cuvette mode

DS-11+ cuvette mode

Enables up to 10 mm pathlength measurements using standard quartz or disposable cuvettes extending the UV-Vis lower detectible limit. The built-in cuvette heater controls temperatures for Kinetics studies. These added features provide a full range of UV-Vis absorbance quantitation in a single instrument.

Extended range

Using the 1 cm cuvette mode enables a lower detection limit 20 times below what is available on the microvolume mode of the DS-11 without the use of fluorescent dyes to gain sensitivity. Measure samples with concentrations as low as 0.04 ng/ul of purified dsDNA or 0.002 mg/ml purified BSA in cuvette mode.

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Kinetics

Create simple kinetics methods measuring two absorbance wavelengths over a user-defined measurement frequency and period.  EasyApps® streamlines the process to create and save custom methods and enables cuvette temperature control between 37°C to 45°C.

Cell culture OD 600

The high-particulate nature of bacterial cell cultures benefit from a longer measurement pathlength. The particulates in these samples scatter light, and a 10 mm pathlength provides a longer cross-section of the sample for the spectrophotometer’s light beam to travel through. This ensures the highest degree of accuracy and reproducibility when measuring these types of samples.

Unmatched connectivity

Built-in Wi-Fi, Ethernet and USB allows easy export of data you want in the format you need. Email a .csv or color .jpg of your results, save data to LIMS or network drives, print results or graphs to network and label printers and more !

Color choices

The DS-11 FX Series are available in four color choices :

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Specifications

Spectrophotometer microvolume mode (DS-11, DS-11+, DS-11 FX, DS-11 FX+)

Minimum sample size0.5 µl
Pathlength0.5 mm (auto ranging to 0.02 mm)
Light sourcePulsed Xenon flash lamp
Detector type2,048 element CCD
Wavelength range190-840 nm
Wavelength accuracy0.5 nm
Spectral resolution1.5 nm (FWHM at Hg 253.7 nm)
Absorbance precision0.015 AU (1 cm), or 1%, whichever is greater
Absorbance accuracy1.5% at 0.75 AU at 260 nm
Absorbance range0.015 – 750 (1 cm equivalent)
Detection limit0.04 mg/ml BSA; 0.75 ng/µl dsDNA
Maximum concentration1,125 mg/ml BSA; 37,500 ng/µl dsDNA
Lower sample surface material of construction303 stainless steel and sapphire window
Upper sample surface material of construction303 stainless steel and quartz fiber

Spectrophotometer cuvette mode (DS-C, DS-11+, DS-11 FX+)

Beam height8.5 mm
Heating37-45°C +/- 0.5 °C
Pathlength10, 5, 2, 1, 0.5, 0.2, 0.125 mm
Absorbance range0.0008-1.5 AU (1 cm)
Detection limit0.002 mg/ml BSA; 0.04 ng/µl dsDNA (1 cm)
Maximum concentration75 ng/µl dsDNA (1 cm)
Light sourcePulsed Xenon flash lamp
Detector type2048 element CCD
Wavelength range190-840 nm
Wavelength accuracy0.5 mm
Spectral resolution1.5 nm (FWHM at Hg 253.7 nm)
Absorbance precision0.015 AU (1 cm), or 1%, whichever is greater
Absorbance accuracy1.5% at 0.75 AU at 260 nm

Fluorometer mode (DS-11 FX, DS-11 FX+ and FX module)

Light sourcesUV LED (~375 nm), Blue LED (~470 nm),
Green LED (~525 nm), Red LED (~635 nm) 
Excitation filtersUV: 361-389 nm, Blue: 442-497 nm,
Green: 490-558 nm, Red: 613-662 nm
Emission filters435-485 nm, 514-567 nm, 565-650 nm, 665-740 nm
DetectorsPhotodiode, detection range 300-1,000 nm
Tube type0.5 ml Real-Time thin-wall PCR tube (polypropylene)

Onboard controller (no PC required)

Operating systemCustom Android™ OS
CPUTI OMAP 1.5 GHz Dual Core ARM Processor
Display1,280 x 800 high definition color display
User inputTouch screen with swipe and pinch motion; glove compatible
Internal storage32 GB flash drive
Connectivity3 USB ports for printer, barcode reader and USB drive
NetworkingEthernet and Wi-Fi

General

ColorsArctic White, Brazilian Blue, Fire Red, Tungsten Silver
Measurement time for spectrophotometry2 seconds
Measurement time for fluorescence2 seconds
Footprint20 cm x 33 cm 
Weight2 kg 
Operating voltage12 VDC
Operating power consumption10W; max 30W
ApprovalsCE, UL/CSA, FCC and Japan CAB
Manufacture locationUSA

Applications

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DS-11 FX key features

  •   Intuitive app Icons for quick recognition
  •   Familiar Android navigation touch gestures and functional icons
  •   Fluid pinch, zoom glove compatible touch interface
  •   AutoRun option for streamlined operation
  •   Optimized screens for reports and graph overlays
  •   Password protected accounts for project and data management
  •   Absorbance represented as 1 cm equivalent for all apps- no confusion between applications
  •   Sample name field is key-focused for easy barcode reader use
  •   All data is automatically archived and easily exported in tab-delimited format

Absorbance :

  • Separate preconfigured apps for optimized workflow
  • Preconfigured and user defined factors for ssDNA ensures appropriate concentration calculations
  • High absorbance capability: up to 37,500 ng/ul for dsDNA
  • 260/280 and 260/230 ratios displayed
  • 11 Preconfigured dyes and fluorophores for Microarray
  • Option to add an unlimited number of new dyes for quick recall in Microarray

Fluorescence :

  • Preconfigured commonly used Qubit®, Quant-it™ and Quantifluor® assays
  • Easy configuration of additional assays
  • Single or multi-replicate standard curve options
  • User control of  line fit, trend line analysis, subtraction of blank
  • User control of assay and reported sample concentration units
  • Save and re-use curves and lists of standard concentrations

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Resources