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CleanNGS DNA & RNA Cleanup For NGS Library Construction [PROMO]

Reference: CNGS0050

35% OFF ALL CLEANNGS KITS

The CleanNGS kit offers a highly efficient magnetic bead based cleanup system for the purification of both DNA and/or RNA for Next-Generation Sequencing workflows.

Features :

  • Designed for both DNA and RNA purification
  • Ideal for (double-sided) size selection for Next-Generation Sequencing
  • Efficient removal of unincorporated dNTPs, primers, primer dimers and other contaminants
  • No centrifugation or filtration

Because of the multiple possible packaging options of this product, the price may vary. Please log in to see the different ordering options.

DNA and RNA clean-up for next generation sequencing library construction

Since its introduction in 2005, Next Generation Sequencing (NGS) opened many doors in the fields of translational genomics and molecular diagnostics by massive parallel decoding of DNA or RNA fragments. To generate high quality NGS data, preparation of pure DNA or RNA of a specific length is one of the key process steps. We offer our CleanNGS for library cleanup and size selection to make this process simple and reliable.

CleanNGS's special buffer formula ensures optimal size selection for NGS libaries and the high quality magnetic beads allow faster separations and better RNA/DNA recovery. CleanNGS is produced RNase free, which makes it an ideal solution for all downstream RNA or DNA NGS experiments.

CleanNGS is suited for cleanup in between library prepping steps, after library prepping and for size selection in next-generation sequencing or sanger sequencing. It is also a highly efficient cleanup method to use before other techniques such as PCR, cloning or CRISPR-Cas9 to improve the quality of your results. CleanNGS purification reagent provides maximum flexibility allowing for left, right or double-sided size selection by easily adjusting the sample to CleanNGS volume ratio(s).

Based on CleanNA’s proprietary chemistry CleanNGS removes, salts, primers, primer-dimers and dNTPs, while DNA and/or RNA fragments are selectively bound to the magnetic particles based on their size. Purified DNA and RNA is eluted off the magnetic particles using water or a low salt buffer and can be used directly for downstream applications. The protocol can be adapted to your current liquid handling workstation (e.g. Hamilton, Beckman, Agilent, Caliper, Perkin Elmer, Tecan and Eppendorf) utilizing your current protocol as well as being used manually.

Workflow

For double sided size selection, first add CleanNGS reagent with magnetic beads in a certain volume ratio. Separate the large DNA or RNA fragments from the solution with a magnetic plate and add more CleanNGS reagent to the supernatant to clean up the small DNA fragments and inhibitors. After two washing steps, the purified DNA or RNA is eluted.

Samples purified with CleanNGS system are ready to be used in the following applications :

  • Next-Generation Sequencing
  • PCR
  • Genomic DNA cleanup
  • RNA cleanup
  • Fragment analysis
  • Microarrays
  • Restriction enzyme cleanup
  • Cloning
Product number Description Number of reactions
Storage conditions
CNGS0050D CleanNGS – 50 ml 2,777 * 4-8⁰C
DO NOT FREEZE
CNGS0500D CleanNGS – 500 ml 27,777 *
* Number of reactions is based on a typical 10 μl PCR reaction volume. Volume of CleanNGS to be used per reaction = 1.8X the sample volume.

App Note NGS Lib Purification

Download (328.89KB)

App Note NGS Lib Size Selection

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